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MABS1976-100UG Anti-Del-1 Antibody, clone D345

Detects Del-1 using this Anti-Del-1, clone D345, Cat. No. MABS1976, is a mouse monoclonal antibody that detects Developmentally-regulated endothelial cell locus 1 protein (Del-1) and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition assay, & Western Blot.

Anti-Del-1 Antibody, clone D345 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: EDIL3, DEL1 UniProt Number: O43854
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MABS1976-100UG
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, FC, IH(P), Inhibition, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1976-100UG
      DescriptionAnti-Del-1 Antibody, clone D345
      Alternate Names
      • EGF-like repeat and discoidin I-like domain-containing protein 3
      • Developmentally-regulated endothelial cell locus 1 protein
      • Integrin-binding protein DEL1
      Background InformationEGF-like repeat and discoidin I-like domain-containing protein 3 (UniProt: O43854; also known as Developmentally-regulated endothelial cell locus 1 protein, Integrin-binding protein DEL1, Del-1) is encoded by the EDIL3 (also known as DEL1) gene (Gene ID: 10085) in human. Del-1 is a calcium-dependent glycoprotein that is secreted by endothelial cells and is shown to be expressed in a developmentally restricted pattern during embryogenesis. In adult tissues it is expressed in endothelial cells and in macrophages. Del-1 is reported to prevent apoptosis in endothelial cell subjected to TNF alpha, etoposide, and anoikis treatment. This anti-apoptotic effect is shown to be mediated specifically through binding of integrin alphav beta3 by the RGD motif. Del-1 expression is reported to be inversely related to that of IL-17 that is shown to inhibit Del-1 via a GSK-3beta and C/EBPb-dependent mechanism. This inhibitory action can be reversed at the PI3K/Akt signaling induced by D-resolvins. It serves as an endothelial cell-secreted homeostatic factor that regulates local inflammation in several tissues. It is reported to interfere with beta2-integrin-dependent inflammatory cell adhesion to endothelium and restricts transendothelial migration of neutrophils. Del-1 is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off in the mature form. It has three EGF-like domains in its N-terminal region. EGF-like domains 2 and 3 form a rigid rod via an interdomain calcium ion binding site, while the long linker between EGF1 and EGF2 lends considerable flexibility to EGF1. Del-1 also has discoidin I-like domains at the C-terminal region that mediate its binding to phosphatidylserine. Two isoforms of Del-1 have been described that are produced by alternative splicing. (Ref.: Dasgupta, SK et al. (2012). Circulation 125 (13); 1664-1672; Wang, Z., et al. (2012). Surgery 151(2); 296-305; Maekawa, T et al. (2015). Nat. Commun. DOI: 10.1038/ncomms9272).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Applications
      ApplicationDetects Del-1 using this Anti-Del-1, clone D345, Cat. No. MABS1976, is a mouse monoclonal antibody that detects Developmentally-regulated endothelial cell locus 1 protein (Del-1) and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition assay, & Western Blot.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      • Inhibition
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Del-1 in human cerebral cortex tissue sections.

      ELISA Analysis: A representative lot detected Del-1 in ELISA applications (Dasgupta, S.K., et. al. (2012). Ciculation. 125(13):1664-72; Maekawa, T., et. al. (2015). Nat Commun. 6:8272).

      Inhibition Analysis: A representative lot inhibited the uptake of BODIPY-labeled platelet microparticles by endothelium. (Dasgupta, S.K., et. al. (2012). Ciculation. 125(13):1664-72).

      Western Blotting Analysis: A representative lot detected Del-1 in Western Blotting applications (Dasgupta, S.K., et. al. (2012). Ciculation. 125(13):1664-72).

      Flow Cytometry Analysis: A representative lot detected Del-1 in Flow Cytometry applications (Dasgupta, S.K., et. al. (2012). Ciculation. 125(13):1664-72).
      Biological Information
      ImmunogenGST-tagged full length human recombinant Developmentally-regulated endothelial cell locus 1 protein (Del-1).
      CloneD345
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone D345 detects human Developmentally-regulated endothelial cell locus 1 protein (Del-1).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EDIL3
      • DEL1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight53.76 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human pancreas tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Del-1 in human pancreas tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1976-100UG 04054839573095