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324393 Anti-Dopamine D₂ Receptor Rabbit pAb

Anti-Dopamine D₂ Receptor, rabbit polyclonal, specifically recognizes the dopamine D2 receptor in rat brain. Has minimal reactivity with the D2S receptor. It is validated for use in ELISA, WB, & IHC.

Anti-Dopamine D₂ Receptor Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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324393
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, RRbPolyclonal Antibody

Pricing & Availability

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324393-100UL
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      Description
      OverviewRecognizes the dopamine D2 receptor in rat brain. Does not cross-react with other dopamine receptors and exhibits minimal cross-reactivity with the D2S short receptor.
      Catalogue Number324393
      Brand Family Calbiochem®
      References
      ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
      Product Information
      FormLyophilized
      FormulationUndiluted serum.
      Positive controlRat brain
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesFrozen Sections Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1:800)
      Frozen Sections (1:5000, see application references)
      Application CommentsThis antibody is highly specific for the immunizing peptide sequence and is suitable for detection of both the D2S and D2L dopamine receptors in human and rat samples. This antibody has also been reported to work for ELISA. For immunoblotting, a 1:800 dilution of the reconstituted antiserum detects two bands at ~48 and ~51 kDa in an analysis using whole rat brain homogenates. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For frozen sections, a 1:5000 dilution of the reconstituted antiserum demonstrates specific staining of rat brain sections including the plasma membrane in the cortex, caudate putamen, globus pallidus, thalamic nucleus, supra optic nucleus, and the dentate gyrus. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (CAARRAQELEME) corresponding to amino acids 272-282 of human D₂ dopamine receptor
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      324393-100UL 04055977216523

      Documentation

      Anti-Dopamine D₂ Receptor Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Dopamine D₂ Receptor Rabbit pAb Certificates of Analysis

      TitleLot Number
      324393

      References

      Reference overview
      Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-July-2007 RFH
      ApplicationELISA (see comments)
      Immunoblotting (1:800)
      Frozen Sections (1:5000, see application references)
      DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa dopamine D2 receptor protein.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (CAARRAQELEME) corresponding to amino acids 272-282 of human D₂ dopamine receptor
      IsotypeIgG
      Specieshuman, rat
      Positive controlRat brain
      FormLyophilized
      FormulationUndiluted serum.
      PreservativeNone
      CommentsThis antibody is highly specific for the immunizing peptide sequence and is suitable for detection of both the D2S and D2L dopamine receptors in human and rat samples. This antibody has also been reported to work for ELISA. For immunoblotting, a 1:800 dilution of the reconstituted antiserum detects two bands at ~48 and ~51 kDa in an analysis using whole rat brain homogenates. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For frozen sections, a 1:5000 dilution of the reconstituted antiserum demonstrates specific staining of rat brain sections including the plasma membrane in the cortex, caudate putamen, globus pallidus, thalamic nucleus, supra optic nucleus, and the dentate gyrus. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
      Toxicity Standard Handling
      ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
      Application referencesFrozen Sections Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.