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07-1708 Anti-FOXH1 Antibody

Anti-FOXH1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXH1 also known as Forkhead activin signal transducer, TGF-beta/activin signal transduce & has been validated in ICC.

Anti-FOXH1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: FAST-1, FAST1, FAST2, Fast-1, Fast-2, hFAST-1 UniProt Number: O75593
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07-1708
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1708
      DescriptionAnti-FOXH1 Antibody
      Alternate Names
      • Forkhead activin signal transducer 1
      • Forkhead activin signal transducer 2
      • TGF-beta/activin signal transducer
      • forkhead activin signal transducer-1
      • forkhead box H1
      Background InformationForkhead box H1 (FOXH1) is a member of the HNF–3FKH family of transcriptional activators. FOXH1 is a winged helix with two loops on the C-terminal side expressed in all human tissue. FOXH1 is an embryonic transcription regulator and is an essential transcriptional co-activator that binds with SMAD2 to activate activin, and therefore induce the gooseciod (GSC) promoter. After FOXH1 binds to SMAD4, activin response element is activated and TGF-β is stimulated. FOXH1 also interacts with NKX2-5 to form an essential component of anterior heart field development. FOXH1 is associated with complex brain malfunctions due to incomplete cleavage of the prosencephalon, and in developmental delays resulting in facial abnormalities such as cyclopia and severe cleft lip or palate.
      References
      Product Information
      FormatAffinity Purified
      Control
      • NIH/3T3, HeLa, and A431 cells.
      PresentationPurified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-FOXH1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXH1 also known as Forkhead activin signal transducer, TGF-beta/activin signal transduce & has been validated in ICC.
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenSynthetic to peptide corresponding to FOXH1.
      HostRabbit
      SpecificityThis antibody recognizes FOXH1.
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman. Predicted to react with mouse based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FAST-1
      • FAST1
      • FAST2
      • Fast-1
      • Fast-2
      • hFAST-1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION:Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element). Ref.1

      SUBUNIT: Interacts with the MH2 domains of SMAD2 and SMAD3. Ref.1

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Ubiquitous. Ref.1

      DOMAIN:The FM region is required for binding SMAD2/SMAD4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated SMAD2 that is in an activated SMAD complex By similarity.

      Sequence similarities Contains 1 fork-head DNA-binding domain.
      Molecular Weight38 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by immunocytochemistry on NIH/3T3, HeLa, and A431 cells.

      Immunocytochemistry:
      1:500 dilution of this antibody was used to detect FOXH1 in NIH/3T3, HeLa and A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-1708 04053252324376