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AB1954-25UL Anti-Fibronectin (rabbit polyclonal)

Anti-Fibronectin Antibody detects level of Fibronectin & has been published & validated for use in ELISA & WB.

Anti-Fibronectin (rabbit polyclonal) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: FN1, DKFZp686O13149, DKFZp686H0342, DKFZp686I1370, MSF, FN, CIG, DKFZp686F10164, LETS, FINC UniProt Number: P02751
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AB1954-25UL
25 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MELISA, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1954-25UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Fibronectin (rabbit polyclonal)
      Alternate Names
      • Cold-insoluble globulin
      • fibronectin 1
      • migration stimulating factor
      • migration-stimulating factor
      Background InformationFibronectin (FN) is involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Western Blot: Normal rat serum
      PresentationPurified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Fibronectin Antibody detects level of Fibronectin & has been published & validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Biological Information
      ImmunogenPurified rat plasma fibronectin.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThe antibody reacts with the 262 kDa fibronectin protein.
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteReacts with mouse and rat. Other species have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
      Gene Symbol
      • FN1
      • DKFZp686O13149
      • DKFZp686H0342
      • DKFZp686I1370
      • MSF
      • FN
      • CIG
      • DKFZp686F10164
      • LETS
      • FINC
      Purification MethodAntigen Affinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
      SIZE: 2386 amino acids; 262607 Da
      SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
      TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
      PTM: Sulfated.
      SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
      Molecular Weight262 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on normal rat serum.

      Western Blot Analysis:
      0.05 μg/mL - 1 μg/mL antibody detected fibronectin in 15 μL of normal rat serum.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB1954-25UL 04054839350481