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AB2302-25UG Anti-GAPDH

Anti-GAPDH Antibody detects level of GAPDH & has been published & validated for use in WB.

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AB2302-25UG
25 μg  
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      Description
      Catalogue NumberAB2302-25UG
      DescriptionAnti-GAPDH
      Alternate Names
      • glyceraldehyde 3-phosphate dehydrogenase
      • aging-associated gene 9 protein
      • glyceraldehyde-3-phosphate dehydrogenase
      Background InformationGlyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK293 cell lysate
      PresentationPurified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GAPDH Antibody detects level of GAPDH & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot (SNAP ID) Analysis: 0.125 µg/ml from a previous lot detected GAPDH on 10 µg of NIH/3T3 and PC12 cell lysates.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the WARS interacting region of human GAPDH.
      EpitopeWARS interacting region
      ConcentrationPlease refer to lot specific datasheet.
      HostChicken
      SpecificityThis antibody recognizes GAPDH.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Horse
      • Canine
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Predicted to react with equine and canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq].
      Gene Symbol
      • GAPDH
      • G3PDH
      • GAPD
      • G3PD
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.

      SIZE: 335 amino acids; 36053 Da

      SUBUNIT: Homotetramer. Binds PRKCI.

      SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane.

      SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
      Molecular Weight~ 38 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 and PC12 cell lysate.

      Western Blot Analysis: 0.0625 µg/ml of this antibody detected GAPDH on 10 µg of HEK293 and PC12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB2302-25UG 04054839342622

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