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MABN1871-25UL Anti-HCN4 Antibody, clone PG2-1A4

Anti-HCN4, clone PG2-1A4 Antibody, Cat. No. MABN1871, is a highly specific rat monoclonal antibody that targets murine HCN4 and has been tested for use in immunofluorescence, Immunohistochemistry, Electron Microscopy, and Western Blotting.

Anti-HCN4 Antibody, clone PG2-1A4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Hcn4, Bcng3 UniProt Number: O70507
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MABN1871-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIF, IHC, EM, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1871-25UL
      DescriptionAnti-HCN4 Antibody, clone PG2-1A4
      Alternate Names
      • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
      • Brain cyclic nucleotide-gated channel 3
      • BCNG-3
      Background InformationPotassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (UniProt: O70507; also known as Brain cyclic nucleotide-gated channel 3, BCNG-3, HCN4) is encoded by the Hcn4 (also known as Bcng3) gene in murine species. HCN4 is a homo- or hetero-tetrameric multi-pass membrane protein that is detected in a subset of elongated cells in taste buds. It acts as a hyperpolarization-activated ion channel with very slow activation and inactivation. It exhibits weak selectivity for potassium over sodium ions. HCN4 is reported to contribute to the native pacemaker currents in heart that regulates the rhythm of heart beat. It may also contribute to the native pacemaker currents in neurons. HCN4 is activated by cAMP and cAMP binding causes a conformation change that leads to the assembly of an active tetramer and induces channel opening. This channel may mediate responses to sour stimuli.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-HCN4, clone PG2-1A4 Antibody, Cat. No. MABN1871, is a highly specific rat monoclonal antibody that targets murine HCN4 and has been tested for use in immunofluorescence, Immunohistochemistry, Electron Microscopy, and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry
      • Electron Microscopy
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A representative lot detected HCN4 in Immunofluorescence applications (Cichy, A., et. al. (2015). J Neurosci. 35(9):4025-39).

      Immunohistochemistry Analysis: A 1:100-500 dilution from a representative lot detected HCN4 in Mouse (C57BL/6) retina tissues (Courtesy of Prof. Dr. Frank Müller, Institute of Complex Systems, Cellular Biophysics (ICS-4), Forschungszentrum Jülich, Germany).

      Electron Microscopy Analysis: A representative lot detected HCN4 in Electron Microscopy applications (Mataruga, A., et. al. (2007). J Comp Neurol. 502(6):1123-37).

      Immunohistochemistry Analysis: A representative lot detected HCN4 in Immunohistochemistry applications (Cichy, A., et. al. (2015). J Neurosci. 35(9):4025-39; Mataruga, A., et. al. (2007). J Comp Neurol. 502(6):1123-37).

      Western Blotting Analysis: A representative lot detected HCN4 in Western Blotting applications (Muller, F., et. al. (2003). Eur J Neruosci. 17(10):2084-96).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 70 amino acids from the C-terminal region of murine Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4).
      ClonePG2-1A4
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone PG2-1A4 detects murine Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4). It targets an epitope within 70 amino acids from the C-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Hcn4
      • Bcng3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight127.41 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.
      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1871-25UL 04054839514869