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MABE1109 Anti-HHV-8 LNA-1 Antibody, clone LN53

Anti-HHV-8 LNA-1, clone LN53, Cat. No. MABE1109, is a highly specific rat monoclonal antibody that targets human KSHV/HHV8 and simian RFHV Orf73 latency-associated nuclear antigen (LANA or LNA) and has been tested in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitati

Anti-HHV-8 LNA-1 Antibody, clone LN53 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: ORF73 UniProt Number: Q9DUM3, Q9QR71
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MABE1109
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Rhesus MacaqueELISA, FC, ICC, IHC, IPRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1109
      DescriptionAnti-HHV-8 LNA-1 Antibody, clone LN53
      Alternate Names
      • HHV-8/KSHV Orf73 LANA
      • HHV-8/KSHV Orf73 LNA
      • HHV-8/KSHV Orf73 LNA-1
      • HHV-8/KSHV Orf73 latency-associated nuclear antigen
      • HHV-8/KSHV Orf73 latent nuclear antigen
      • HHV-8/KSHV Orf73 latent nuclear antigen-1
      Background InformationHuman herpesvirus-8//Kaposi's sarcoma-associated herpesvirus (HHV-8/KSHV) and macaque retroperitoneal fibromatosis herpesvirus (RFHV) were originally identified in in AIDS patients in association with Kaposi's sarcoma (KS) and in retroperitoneal fibromatosis (RF) tumor lesions of macaques with simian AIDS, respectively. The most prominent protein expressed in cells latently infected with KSHV and RFHV is the orf73 gene product known as the latency-associated nuclear antigen (LANA or LNA). LANA is a nuclear protein that functions to ensure the maintenance of the viral genome by tethering the viral episomal DNA to host cell chromosomes. LANA also regulates the cellular transcription program in host cells through interaction with a number of cellular proteins, including transcriptional regulators and known tumor suppressors, p53 and pRB. In addtion, LANA directly influences the viral transcription program and helps maintain the latent state of the virus by inhibiting viral replication. KSHV LANA consists of a serine- and proline-rich N-terminal domain with a nuclear localization signal (NLS), a chromatin-binding motif (CBM), and domains responsible for interaction with the transcription regulators, mSin3 complex and Sp1. The central domain contains several highly repetitive acidic regions that vary in length and are responsible for the size variation of LANA proteins from different KSHV isolates that can range from 1003 to 1162 amino acids. The proline-rich C-terminal third domain contains another NLS and is responsible for LANA dimerization and binding to the terminal repeats (TR) of the viral genomic DNA. The C-terminal domain is responsible for interaction with tumor suppressors pRB and p53.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-HHV-8 LNA-1, clone LN53, Cat. No. MABE1109, is a highly specific rat monoclonal antibody that targets human KSHV/HHV8 and simian RFHV Orf73 latency-associated nuclear antigen (LANA or LNA) and has been tested in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitati
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesELISA Analysis: A representative lot detected HHV-8 LNA-1 recombinant constructs containing the repetitive region a.a. 803-929 (Kellam, P., et al. (1999). J. Virol. 73(6): 5149 5155).

      Flow Cytometry Analysis: A representative lot detected HHV-8 LNA-1 immunoreactivity in BCP-1, BC-3, and HBL-6 cells, but not in Daudi or Ramos cells (Kellam, P., et al. (1999). J. Virol. 73(6): 5149 5155).

      Immunocytochemistry Analysis: A representative lot detected the transiently expressed KHSV ORF73 (HHV-8 LANA or LNA-1) as well as rhesus macaque retroperitoneal fibromatosis herpesvirus ORF73 (RFHV LANA) in the nuclei of transfected COS cells by fluorescent immunocytochemistry. No reactivity was detected toward pig-tailed macaque MneRV2 or rhesus macaque RRV LANA proteins (Burnside, K.L., et al. (2006). Virology. 354(1):103-115).

      Immunocytochemistry Analysis: A representative lot detected HHV-8 LNA-1-positive nuclear bodies in primary effusion lymphoma (PEL) cell line BCP-1 by fluorescent immunocytochemistry, but not in Daudi or Ramos cells (Kellam, P., et al. (1999). J. Virol. 73(6): 5149 5155).

      Immunohistochemistry Analysis: A representative lot detected RFHVMn LANA immunoreactivity in the nuclei of retroperitoneal fibromatosis (RF) tumor cells by immunohistochemistry staining of formalin-fixed, paraffin-embedded RF biopsies from an SIV-infected pig-tailed macaque. No immunoreactivity was detected using normal macaque jejunum tissue sections (Burnside, K.L., et al. (2006). Virology. 354(1):103-115).

      Immunohistochemistry Analysis: Representative lots immunostained cells latently infected by HHV-8 by immunohistochemistry staining of paraffin-embedded patch, plaque, and nodular Kaposi s sarcoma (KS), multicentric Castleman s disease (MCD), and primary effusion lymphoma (PEL) tissue sections (Codish, S., et al. (2000). Am. J. Hematol. 65(4):310-314; Dupin, N., et al. (2000). Blood. 95(4):1406-1412; Dupin, N., et al. (1999). Proc. Natl. Acad. Sci. U. S. A. 96(8):4546-4551).

      Immunohistochemistry Analysis: A representative lot detected nuclear HHV-8 LNA-1 immunoreactivity in cells within the nodular Kaposi s sarcoma (KS) lesion, but not in the surrounding dermis by immunohistochemistry staining of paraffin-embedded classical KS tumor sections (Kellam, P., et al. (1999). J. Virol. 73(6): 5149 5155).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated 220/230 kDa HHV-8 LNA-1 from primary effusion lymphoma (PEL) cell line BC-3, but not from Ramos cells (Kellam, P., et al. (1999). J. Virol. 73(6): 5149 5155).

      Western Blotting Analysis: A representative lot detected the transiently expressed KHSV ORF73 (HHV-8 LANA or LNA-1) as well as rhesus macaque retroperitoneal fibromatosis herpesvirus ORF73 (RFHV LANA) in lysate from transfected COS cells. No reactivity was detected toward pig-tailed macaque MneRV2 or rhesus macaque RRV LANA proteins (Burnside, K.L., et al. (2006). Virology. 354(1):103-115).

      Western Blotting Analysis: A representative lot detected the 220/230 kDa HHV-8 LNA-1 doublet in lysate from primary effusion lymphoma (PEL) cell line BC-3, but not from Ramos cells (Kellam, P., et al. (1999). J. Virol. 73(6): 5149 5155).
      Biological Information
      ImmunogenAffinity-purified GST fusions of HHV-8 LNA-1 C-terminal fragments (Kellam, P., et al. (1999). J. Virol. 73(6): 5149 5155).
      CloneLN53
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone LN53 recognizes repetitive glutamic acid structural motifs EQEQE and EPEPE found in the Glu-rich repeat region of human Kaposi's sarcoma-associated herpesvirus (KSHV or HHV-8) and simian retroperitoneal fibromatosis herpesvirus (RFHV) Orf73 latency-associated nuclear antigen (LANA or LNA), respectively.
      IsotypeIgG2cκ
      Species Reactivity
      • Human
      • Rhesus Macaque
      Species Reactivity NoteHuman KSHV (HHV8) and simian RFHV.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ORF73
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight116/135 kDa (KSHV LNA) and 118 kDa (RFHV LNA) prediced, ~220/230 kDa doublet (KSHV) and ~240 kDa doublet (RFHV) reported (Burnside, K.L., et al. (2006). Virology. 354(1):103-115; Kellam, P., et al. (1999). J. Virol. 73(6): 5149 5155). The larger-than-predicted apparent molecular weight is most likely caused by the internal acidic repeat region sequence (Rainbow, L., et al. (1997). J. Virol. 71(8):5915-5921).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in BCBL-1 cells.

      Immunocytochemistry Analysis (ICC): 1 µg/mL of this antibody immunostained HHV-8 LNA-1-positive nuclear bodies in Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8- (KSHV/HHV8-) infected BCBL-1 lymphoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1109 04054839034985

      Documentation

      Anti-HHV-8 LNA-1 Antibody, clone LN53 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-HHV-8 LNA-1 Antibody, clone LN53 Certificates of Analysis

      TitleLot Number
      Anti-HHV-8 LNA-1, clone LN53 - 3268881 3268881
      Anti-HHV-8 LNA-1, clone LN53 - 3484023 3484023
      Anti-HHV-8 LNA-1, clone LN53 - 3707312 3707312
      Anti-HHV-8 LNA-1, clone LN53 - 3850506 3850506
      Anti-HHV-8 LNA-1, clone LN53 - 4070061 4070061
      Anti-HHV-8 LNA-1, clone LN53 - 4122574 4122574
      Anti-HHV-8 LNA-1, clone LN53 - 4171246 4171246
      Anti-HHV-8 LNA-1, clone LN53 - 4239684 4239684
      Anti-HHV-8 LNA-1, clone LN53 -Q2724502 Q2724502
      Anti-HHV-8 LNA-1, clone LN53 Monoclonal Antibody 3111501
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