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MABC1138-25UG Anti-HPCAL1 Antibody, clone 5A5

Detects Hippocalcin-like protein 1 using this mouse monoclonal Anti-HPCAL1, clone 5A5, Cat. No. MABC1138, tested for use in Western Blotting, Immunohistochemistry (Paraffin), ELISA and Immunocytochemistry.

Anti-HPCAL1 Antibody, clone 5A5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: HPCAL1, BDR1 UniProt Number: P37235
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MABC1138-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P), ELISA, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1138-25UG
      DescriptionAnti-HPCAL1 Antibody, clone 5A5
      Alternate Names
      • Hippocalcin-like protein 1
      • Calcium-binding protein BDR-1
      • HLP2
      • Visinin-like protein 3
      • VILIP-3
      Background InformationHippocalcin-like protein 1 (UniProt: P37235; also known as Calcium-binding protein BDR-1, HLP2, Visinin-like protein 3, VILIP-3) is encoded by the HPCAL1 (also known a BDR1) gene (Gene ID: 3241) in human. HPCAL1 is a lipid-anchor membrane protein of the visinin-like subfamily of neural calcium sensor and is involved in the calcium-dependent regulation of rhodopsin phosphorylation. It is structurally related to Ca2+-myristoyl switch proteins that contain four EF-hand motifs and a covalently attached N-terminal myristoyl group. HPCAL1 is also expressed in Purkinje cells of the cerebellum and hippocampus where it is believed to regulate synaptic plasticity relevant for learning and memory. Clone 5A5 can detect HPCAL1 in formalin-fixed 293 cells and in pancreatic cell lines. (Ref.: Chen, Y., et al. (2014). Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 33(1); 20-27; Li, C., et al. (2016). PLoS One 11(11); e0165921).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Applications
      ApplicationDetects Hippocalcin-like protein 1 using this mouse monoclonal Anti-HPCAL1, clone 5A5, Cat. No. MABC1138, tested for use in Western Blotting, Immunohistochemistry (Paraffin), ELISA and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • ELISA
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected HPCAL1 in Immunocytochemistry applications (Chen, Y., et. al. (2014). Monoclon Antib Immunodiagn Immunother. 33(1):20-7).

      ELISA Analysis: A representative lot detected HPCAL1 in ELISA applications (Chen, Y., et. al. (2014). Monoclon Antib Immunodiagn Immunother. 33(1):20-7).

      Immunohistochemistry (Paraffin) Analysis: A 1:50-250 Dilution from a representative lot detected HPCAL1 in human kidney and human cerebellum tissue sections.
      Biological Information
      ImmunogenNative and denatured human HPCAL1.
      Clone5A5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5A5 specifically detects Hippocalcin-like protein 1 (HPCAL1) in human cells.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HPCAL1
      • BDR1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~22 kDa observed; 22.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Panc-1 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected HPCAL1 in Panc-1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1138-25UG 04054839529580