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MABF3220-25UG Anti- HPV16 Antibody, clone H16.V5

Anti- HPV16, clone H16.V5, Cat. No. MABF3220, is mouse monoclonal antibody that detects HPV16 and is tested for use in and ELISA and Neutralizing Assay.

MABF3220-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3220-25UG
      DescriptionAnti- HPV16 Antibody, clone H16.V5
      Alternate Names
      • Major capsid protein L1
      • HPV-16
      Background InformationMajor capsid protein L1 (UniProt: P03101) is encoded by the L1 gene (Gene ID: 1489082) in Human papillomavirus type 16. The Major Capsid Protein L1 of Human papillomavirus is the primary structural protein responsible for the formation of the viral capsid, that encapsulates the genomic DNA together with Minor Capsid Protein L2. The viral capsid is composed of 360 copies of the L1 protein forming 72 pentamers, that assemble into the icosahedral capsid structure, while the L2 protein localizes along the inner surface of the virion and plays a role in capsid stabilization through interaction with the Major Capsid Protein L1. The L1 protein facilitates the attachment of the virus to the host cell by interacting with heparan sulfate proteoglycans on the cell surface, a critical step for infection. Recombinantly expressed Major Capsid Protein L1 can spontaneously assemble into Virus Like-Particles (VLPs) and were used for the development of neutralizing antibodies that show high degree of Human papillomavirus type-specificity. Based on their oncogenic potential, Human papillomaviruses (HPVs) can be classified as low-risk HPV types, (e.g.: HPV6 and HPV11, the most common serotypes) which do not cause cancer but can lead to skin and genital warts, and high-risk HPV types (e.g.: HPV 16, 18, 31, 33, 35, 45, 52, 58), that are responsible for HPV related cancers. Vaccines targeting the L1 protein, have been developed and are effective in preventing infection with the most oncogenic HPV types by inducing a robust antibody response. (Ref.: Hareza, D. A., (2022) Int. J. Mol. Sci., 23, 1818.; Christensen, N.D., et al. (1990). J Virol. 64:11, 5678-81; Christensen, N.D., et al. (1996). Virology. 224(2):477-86; Brown, M.J., et al. (2014). Clin Vaccine Immunol. 21:4, 587-93)
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in PBS without preservatives
      Quality LevelMQ200
      Applications
      ApplicationAnti- HPV16, clone H16.V5, Cat. No. MABF3220, is mouse monoclonal antibody that detects HPV16 and is tested for use in and ELISA and Neutralizing Assay.
      Key Applications
      • Neutralizing
      • ELISA
      Application NotesTested Applications

      ELISA Analysis: A representative lot of this antibody detected HPV16 protein in ELISA application (Data by Dr. J. Leitsch and with kind permission of ARTES Biotechnology GmbH, Germany. Recombinant HPV L1 antigens used were produced by ARTES Biotechnology GmbH).

      Neutralizing: A representative lot detected HPV16 in Neutralizing application (Christensen, N.D., et al. (1996). Virology. 223(1):174-84; McClements, W.L., et al. (Virology). 289(2):262-8; Dias, D., et al. (2005). Clin Diagn Lab Immunol. 12(8):959-69; Smith, J.F., et al. (2008). Hum Vaccin. 4(2):134-42; Jerajani, K., et al. (2022). J Pharm Sci. 111(11):2983-2997).

      ELISA Analysis: A representative lot detected HPV16 in ELISA application (Christensen, N.D., et al. (1996). Virology. 223(1):174-84; McClements, W.L., et al. (Virology). 289(2):262-8; Smith, J.F., et al. (2008). Hum Vaccin. 4(2):134-42; Panicker, G., et al. (2021). J Immunol Methods. 498:113136; Jerajani, K., et al. (2022). J Pharm Sci. 111(11):2983-2997).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenRecombinant baculovirus-induced HPV-16 L1 Virus Like-Particles (VLPs)
      EpitopeUnknown
      CloneH16.V5
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone H16.V5 is a mouse monoclonal antibody that detects HPV16. It targets an epitope within the Major capsid protein L1. Might show some cross reactivity with HPV11.
      IsotypeIgG2bκ
      Species Reactivity
      • Virus
      Species Reactivity NoteHuman Papillomavirus
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • L1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight56.28 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b .
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3220-25UG 04065272074025