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MABF951-100UL Anti-IL-2R beta (CD122) Antibody, clone TM-beta1

Anti-IL-2R beta (CD122), clone TM-beta1, Cat. No. MABF951, is a rat monoclonal antibody that detects IL-2R beta and has been tested for use in Flow Cytometry, Function assay, Immunocytochemistry, Immunoprecipitation, Neutralizing, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Il2rb UniProt Number: P16297
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MABF951-100UL
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFC, Function Assay, ICC, IP, NEUT, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF951-100UL
      DescriptionAnti-IL-2R beta (CD122) Antibody, clone TM-beta1
      Alternate Names
      • Interleukin-2 receptor subunit beta
      • IL-2 receptor subunit beta
      • IL-2R subunit beta
      • High affinity IL-2 receptor subunit beta
      • p70-75
      • CD122
      Background InformationInterleukin-2 receptor subunit beta (UniProt: P16297; also known as IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, High affinity IL-2 receptor subunit beta, p70-75, CD122) is encoded by the Il2rb gene (Gene ID: 16185) in murine species. IL-2R is a non-covalent dimer of an alpha and a beta subunit. It can exist as a high affinity dimer, an intermediate affinity monomer of beta subunit, and as a low affinity monomer of alpha subunit. IL-2R beta is a single-pass type I membrane protein. The beta subunit is involved in receptor mediated endocytosis and it transduces the mitogenic signals of IL-2. IL-2R beta is synthesized with a signal peptide (aa 1-26), which is cleaved off to produce the mature form that has an extracellular domain (aa 27-240), a transmembrane domain (aa 241-268), and a long cytoplasmic domain (aa 269-539). IL-2R beta contains a WSXWS motif (aa 221-225), which is essential for its proper folding and thereby efficient intracellular transport. It also has a Box 1 motif (aa 281-289) that is required for JAK interaction and/or activation.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2b in PBS without azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-IL-2R beta (CD122), clone TM-beta1, Cat. No. MABF951, is a rat monoclonal antibody that detects IL-2R beta and has been tested for use in Flow Cytometry, Function assay, Immunocytochemistry, Immunoprecipitation, Neutralizing, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Function Assay
      • Immunocytochemistry
      • Immunoprecipitation
      • Neutralizing
      • Western Blotting
      Application NotesNeutralizing Analysis: Freshly isolated mouse splenocytes pretreated with different amounts of MABF951, Anti-IL-2R beta (CD122), clone TM- 1 showed a co-relation of the amount of antibody used vs percent of cells stained with FITC-labeled antibody. In antibody titration, as the concentration of unlabeled anti-IL-2R beta (CD122) is decreased less inhibition is observed as a result.

      Inhibits Activity/Function: A representative lot abolished the binding of IL2 ligand to IL-2R beta (CD122). (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8; Yokoyama, S., et. al. (2009). Proc Natl Acad Sci USA. 106(37):15849-54).

      Flow Cytometry Analysis: A representative lot detected IL-2R beta (CD122) in Flow Cytometry applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).

      Western Blotting Analysis: A representative lot detected IL-2R beta (CD122) in Western Blotting applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated IL-2R beta (CD122) in Immunoprecipitation applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).

      Immunocytochemistry Analysis: A representative lot detected IL-2R beta (CD122) in Immunocytochemistry applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).
      Biological Information
      ImmunogenTART-mB whole cells
      CloneTM-beta 1
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone TM-beta 1 is a rat monoclonal antibody that detects IL-2R beta subunit in murine species.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Il2rb
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight60.54 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF951-100UL 04054839441240