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AB1413-I Anti-Interleukin-1β

This rabbit polyclonal Anti-Interleukin-1 Antibody, Cat. No. AB1413-I is validated for use in Western Blotting for the detection of Interleukin-1 .

Anti-Interleukin-1β MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Il1b UniProt Number: P10749
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AB1413-I
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1413-I
      ReplacesAB1413
      DescriptionAnti-Interleukin-1β
      Alternate Names
      • Interleukin-1 beta
      • IL-1 beta
      • IL-1b
      • IL-1beta
      Background InformationInterleukin-1 beta (UniProt; also known as IL-1 beta, IL-1b, IL-1beta) is encoded by the Il1b gene (Gene ID 16176) in murine species. Interleukin-1 (IL-1) is a cytokine that plays a central role in the regulation of acute and chronic inflammation. Two forms of IL-1, IL-1 and IL-1 , are transcribed from two separate genes and mediate similar, but not completely overlapping, biological effects via the membrane-bound IL-1 type 1 receptor (IL-1R1). Studies utilizing Il1a- and Il1b-knockout mice showed that IL-1 , but not IL-1 , is involved in T-cell-dependent antibody production via antigen-specific T-cell activation. IL-1 binding to the extracellular domain of IL1R1 promotes the recruitment of the IL-1 receptor accessory protein and subsequent signal transduction mediated by the cytoplasmic domains of IL-1R1 and IL1-R2. The IL-1 type 2 receptor (IL1R2) is unable to initiate signaling and only acts as a decoy receptor. Soluble receptors for IL-1 (sIL-1Rs) are produced by proteolytic cleavage of membrane-bound receptors or by alternative splicing and act as natural inhibitors by competing against membrane-bound IL1Rs for IL-1 binding. Murine IL-1 beta is produced with a propeptide sequence (a.a. 1-117), which is removed posttranslationally to yield the mature (a.a. 118-269) protein.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rabbit polyclonal Anti-Interleukin-1 Antibody, Cat. No. AB1413-I is validated for use in Western Blotting for the detection of Interleukin-1 .
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected pro- and mature IL-1beta in 10 µg of lysates from mouse testis tissue and LPS-stimulated RAW264.7 cells, respectively.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a region within the C-terminal half of the mature murine and rat IL-1beta sequence.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detected both the ~17 kDa mature and ~30 kDa pro-form of murine IL-1beta. The corresponding sequence in human IL-1beta differs from that of the immunogen by only one amino acid. Immunogen sequence is not found in IL-1alpha.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Gerbil, Hamster, and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Il1b
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~17/30 kDa observed. 17.39/30.93 kDa (mouse mature/pro-form), 17.32/30.64 kDa (rat mature/pro-form) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected IL-1beta in 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB1413-I 04054839055102