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ABC1442 Anti-K-Ras4A Antibody, Isoform-specific

Detect K-Ras4A using this rabbit polyclonal Anti-K-Ras4A Antibody, Cat. No. ABC1442, validated for use in Western Blotting.

Anti-K-Ras4A Antibody, Isoform-specific MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: KRAS, KRAS2, RASK2 UniProt Number: P01116
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ABC1442
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC1442
      DescriptionAnti-K-Ras4A Antibody, Isoform-specific
      Alternate Names
      • GTPase KRas, isoform 2A
      • c-K-ras, isoform 2A
      • K-Ras4A
      • c-Ki-ras, isoform 2A
      • K-Ras 2, isoform 2A
      • Ki-Ras, isoform 2A
      Background InformationGTPase KRas (UniProt P01116; also known as c-K-ras, c-Ki-ras, K-Ras 2, Ki-Ras) is encoded by the KRAS (also known as KRAS2, RASK2) gene (Gene ID 3845) in human. Ras proteins are small GTPases controlling cellular pathways that signal for growth, proliferation, and differentiation. Mammalian genomes harbor three Ras genes (HRAS, NRAS, and KRAS) that encode four GTPases, two of which (K-Ras4A and K-Ras4B) are transcribed from the KRAS gene via two alternative fourth exons. Ras proteins share nearly identical sequence homology in sequence encoded by exons 1-3 (a.a. 1-165), while diverge in their C-terminal 24-a.a. hypervariable region (HVR) encoded by exon 4. The first 165 amino acids form the catalytic G domain that mediates GDP/GTP binding and association with effectors, exchange factors, and GTPase-activating proteins (GAPs), while the C-terminal HVR is needed for Ras membrane localization, a process necessary for Ras function. In addition to the removal of initiator methionine (Met1), Ras proteins are posttranslationally modified by farnesylation of Cys186, followed by proteolytical cleavage their C-terminal end three amino acids (a.a. 187-189) and carboxyl methylation of the newly formed C-terminal prenylcysteine. H-Ras, N-Ras, and K-Ras4A are further palmitoylated on cysteine residue(s) near the C-terminus (Cys181/Cys184 in H-Ras, Cys181 in N- and K-Ras), while K-Ras4B lacks a palmitoylation site. Palmitoylation is required for targeting H- and N-Ras to the plasma membrane (PM), while K-Ras4A and K-Ras4B contain a series of lysines that constitute a polybasic region (PBR) capable of interacting with the inner leaflet of the PM through an electrostatic interaction.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationDetect K-Ras4A using this rabbit polyclonal Anti-K-Ras4A Antibody, Cat. No. ABC1442, validated for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected recombinant K-Ras4A, but not K-Ras4B, H-Ras or N-Ras (Tsai, F.D., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(3):779-784).

      Western Blotting Analysis: A representative lot detected the presence of K-Ras4A in total Ras immunoprecipitates from colorectal carcinoma (HCT116, HT29, and CaCo-2) and T24 urothelial carcinoma cell lysates using a pan-Ras mAb (Cat. No. OP40), clone RAS 10 (Tsai, F.D., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(3):779-784).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a sequence near the C-terminus of human K-Ras4A.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detected recombinant K-Ras4A, but not K-Ras4B, H-Ras or N-Ras (Tsai, F.D., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(3):779-784) by targeting a C-terminal region sequence unique to spliced isoform K-Ras4A (isoform 2A) and not found in spliced isoform K-Ras4B (isoform 2B).
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KRAS
      • KRAS2
      • RASK2
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight21.66 kDa (pro-form, a.a. 1-189) and 21.17 kDa (mature, a.a. 2-186) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected exogenously expressed K-Ras4A GFP fusion protein in 20 µg of lysate from transfected HEK293 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC1442 04054839056277