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MABS2253-100UG Anti-LRP1 α-chain Antibody, clone 8G1

Anti-LRP1 a-chain, clone 8G1, Cat. No. MABS2253, is a mouse monoclonal antibody that detects LRP1 a -chain and is tested for use in ELISA, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

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MABS2253-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2253-100UG
      DescriptionAnti-LRP1 α-chain Antibody, clone 8G1
      Alternate Names
      • Prolow-density lipoprotein receptor-related protein 1
      • Alpha-2-macroglobulin receptor
      • A2MR
      • Apolipoprotein E receptor
      • APOER
      • CD91
      Background InformationProlow-density lipoprotein receptor-related protein 1 (UniProt Q07954; also known as A2MR, Alpha-2-macroglobulin receptor, APER, Apolipoprotein E receptor, CD19. LRP-1) is encoded by the LRP1 (also known as A2MR, APR) gene (Gene ID: 4035) in human. LRP-1 is single pass type I membrane protein that is expressed in most tissues, but is abundant in liver, brain, and lung. It serves as a major VSMC receptor with a crucial role in maintaining vessel wall integrity and its expression in adult smooth muscle cells is shown to be essential for maintaining a contractile phenotype. LRP1 is synthesized with a signal peptide sequence (aa 1-19) and is processed in trans-Golgi network by Furin to generate a 515 kDa a subunit and an 85 kDa b subunit that contains the 100-amino intracellular domain (LRPICD; aa 4445-4544). The a and b subunits are non-covalently linked during LRP1 transport to the cell membrane. LRP1 recognizes and mediates the endocytosis of more than 40 different ligands, including apolipoprotein E (ApoE), APP and amyloid b. It is the primary receptor mediating transport of amyloid b peptides across the blood-brain barrier into circulation, thereby clearing them from the brain. LRP1 is required for early embryonic development and is involved in cellular lipid homeostasis and plasma clearance of chylomicron remnants and activated LRPAP1 (a2-macroglobulin). Excessive copper accumulation in the brain has been linked with reduced LRP1 mediated clearance of amyloid b peptides across the blood brain barrier, which may contribute to complications of Alzheimer's disease.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-LRP1 a-chain, clone 8G1, Cat. No. MABS2253, is a mouse monoclonal antibody that detects LRP1 a -chain and is tested for use in ELISA, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • ELISA
      • Western Blotting
      • Immunoprecipitation
      Application NotesTested Applications

      Immunocytochemistry Analysis: A representative lot detected LRP1 -chain in Immunocytochemistry applications (Craig, J., et al. (2013). PLoS One. 8(7):e70432).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated LRP1 -chain in Immunoprecipitation applications (Ranganathan, S., et al. (2011). J. Biol. Chem.286(35):30535-30541; Boye, K., et al. (2017). Nat. Commun. 8(1):1571).

      Western Blotting Analysis: A representative lot detected LRP1 -chain in Western Blotting applications (Strickland, D.K., et al. (1990). J Biol Chem.;265(29):17401-4; Yamamoto, K., et al. (2013). FASEB J.;27(2):511-21; Yamamoto, K. et al. (2016). Matrix Biol.;56:57-73; Boye, K., et al. (2017). Nat Commun.;8(1):1571).

      ELISA Analysis: A representative lot detected LRP1 -chain in ELISA applications (Ranganathan, S., et al. (2011). J. Biol. Chem. 286(35):30535-3054).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull-length LRP-1 purified from human placenta and 515 kDa band excised from gel and used as immunogen.
      EpitopeN-terminal
      Clone8G1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 8G1 is a mouse monoclonal antibody that detects human LRP-1. It targets an epitope within the a chain in the extracellular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LRP1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight504.61 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in MCF-7 cells.

      Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected LRP1 a-chain in MCF-7 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2253-100UG 04065267027661