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09-872-I-25UG Anti-Methylcytosine dioxygenase TET1

Anti-Methylcytosine dioxygenase TET1, Cat. No. 09-872-I is a rabbit polyclonal antibody that detects Methylcytosine dioxygenase TET1 and is tested for use in Western Blotting.

09-872-I-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue Number09-872-I-25UG
      DescriptionAnti-Methylcytosine dioxygenase TET1
      Alternate Names
      • Methylcytosine dioxygenase TET1
      • CXXC-type zinc finger protein 6
      • Ten-eleven translocation 1 gene protein homolog
      Background InformationMethylcytosine dioxygenase TET1 (UniProt: Q3URK3; also known as CXXC-type zinc finger protein 6, Ten-eleven translocation 1 gene protein homolog) is encoded by the Tet1 (also known as Cxxc6, Kiaa1676) gene (Gene ID: 52463) in murine species. TET1 is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. It is shown to be present in embryonic stem cells. Presence of 5hmC has been reported in the brain, especially in Purkinje neurons. In addition to its role in DNA demethylation, TET1 also plays a more general role in chromatin regulation. It preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. It is involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. TET family consists of three TET enzymes that contain cysteine rich and DSBH domains. TET1 and TET3 also have a CXXC domain, which typically bind unmethylated CpG dinucleotides, but in TET enzymes they bind 5mC and 5hmC.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Methylcytosine dioxygenase TET1, Cat. No. 09-872-I is a rabbit polyclonal antibody that detects Methylcytosine dioxygenase TET1 and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptides corresponding to 12, 14, and 16 amino acids from N-terminal, internal, and C-terminal regions of mouse Methylcytosine dioxygenase TET1.
      Epitopeunknown
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects murine Methylcytosine dioxygenase TET1.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tet1
      • Cxxc6
      • Kiaa1676
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight~ 220 kDa observed; 219.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse Thymus tissue lysates.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Methylcytosine dioxygenase TET1 in Mouse Thymus tissue lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      09-872-I-25UG 04061842378258