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ABC2044-100UG Anti-Muc2-D3

Anti-Muc2-D3, Cat. No. ABC2044, is a rabbit polyclonal antibody that detects Human Mucin-2 protein and is teste for use in Electron Microscopy and Western Blotting.

ABC2044-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABC2044-100UG
      DescriptionAnti-Muc2-D3
      Alternate Names
      • Mucin-2 D3 domain
      Background InformationMucin-2 (UniProt: Q02817; also known as MUC-2, Intestinal Mucin-2) is encoded by the MUC2 (also known as SMUC) gene (Gene ID: 4583) in human. Mucin-2 (MUC2) is a secreted protein that coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. It provides a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. Mucin-2 is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to produce the mature form. It exists as a disulfide-linked homotrimer that can be highly O-glycosylated. It contains four VWFD domains and two VWFC domains. Mucin-2 undergoes proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer that allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria. The numerous O-glycans on the Mucin-2 are reported to serve as nutrients for the bacteria, serve as attachment sites, and probably contribute to the selection of the species-specific colon flora. Reduced levels of Mucin-2 have been linked to the early stage of colorectal cancer development, which is caused by methylation of the MUC2 promoter. This polyclonal antibody detects D3 domain of mucin-2, which is localized in amino acids 858-1259. (Ref.: Wi, D-H., et al. (2021). BMB Rep. 54(7); 344-355; Javitt, G., et al. (2020). Cell. 183(3); 717-729; Javitt, G., et al. (2019). J. Mol. Biol. 431(19); 3740-3752; Johansson, MEV., et al. (2011). Proc. Natl. Acad. Sci. USA. 108(Suppl. 1); 4659-4665).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Muc2-D3, Cat. No. ABC2044, is a rabbit polyclonal antibody that detects Human Mucin-2 protein and is teste for use in Electron Microscopy and Western Blotting.
      Key Applications
      • Western Blotting
      • Electron Microscopy
      Application NotesTested Applications

      Electron Microscopy: A representative lot detected Human Mucin-2 protein in Electron Microscopy applications (Javitt, G., et. al. (2020). Cell.183(3): 717-729).

      Western Blotting Analysis: A representative lot detected Human Mucin-2 protein in Western Blotting applications (Javitt, G., et. al. (2020). Cell. 183(3): 717-729).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 402 amino acids from the D3 domain of human Mucin-2.
      EpitopeN-terminal half
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects D3 domain of Mucin-2. It targets an epitope within D3 domain from the N-terminal half.
      IsotypeIgGκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MUC2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~60 kDa observed for D3 domain; 550.85 kDa calculated for Mucin-2. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with Human Muc2-D3 protein.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Human Muc2-D3 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC2044-100UG 04065270453563