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MAB5384-I-100UG Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 132.38

Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38, Cat. No. MAB5384-I, is a highly specific mouse monoclonal antibody that targets Chondrin Sulfate Protoglycan 4 and has been tested for use in Immunofluorescence and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Cspg4, Ng2 UniProt Number: Q00657
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MAB5384-I-100UG
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MWB, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB5384-I-100UG
      ReplacesMAB5384 and 05-710
      DescriptionAnti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 132.38
      Alternate Names
      • Chondroitin sulfate proteoglycan 4
      • Chondroitin sulfate proteoglycan NG2
      • HSN tumor-specific antigen
      • NG2
      Background InformationChondroitin sulfate proteoglycan 4 (UniProt: Q00657; also known as Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen) is encoded by the Cspg4 (also known as Ng2) gene (Gene ID: 81651) in rat. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane protein that plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration. NG2 chondroitin sulfate proteoglycan is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off in the mature form. (Ref.: Jones, LL et al. (2002). J. Neurosci. 22(7):2792-2803).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing PBS, pH 7.2, containing 0.05% sodium azide.
      Applications
      ApplicationAnti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38, Cat. No. MAB5384-I, is a highly specific mouse monoclonal antibody that targets Chondrin Sulfate Protoglycan 4 and has been tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected NG2 Chondroitin Sulfate Proteoglycan in Rat E16 brain tissuel lysate.

      Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected NG2 Chondroitin Sulfate Proteoglycan in rat brain frozen sections, post-fixed in ethanol.
      Biological Information
      ImmunogenHEK293 cells expressing a truncated integral membrane form of NG2 consisting of amino acids 1592-2222.
      Clone132.38
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 132.38 is a mouse monoclonal antibody that detects Chondroitin sulfate proteoglycan 4 in rat brain tissue.
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteRat, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cspg4
      • Ng2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~260 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected NG2 Chondroitin Sulfate Proteoglycan in NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB5384-I-100UG 04054839510243

      Documentation

      Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 132.38 Certificates of Analysis

      TitleLot Number
      Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 3215962 3215962
      Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 3425417 3425417
      Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 3684697 3684697
      Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 3975474 3975474
      Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 4101918 4101918
      Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 4171292 4171292
      Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 4201226 4201226
      Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 4259764 4259764
      Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 Monoclonal Antibody 3090746