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MABF2127-100UG Anti-Properdin Antibody, clone 3A3E1

This mouse monoclonal Anti-Properdin, clone 3A3E1 Antibody, Cat. No. MABF2127 is tested for use in Western Blotting, ELISA, Immunohistochemistry (Paraffin)for the detection of Properdin. Inhibitis the functionn of properdin by inhibiting its binding to C3b.

Anti-Properdin Antibody, clone 3A3E1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CEP, PFC UniProt Number: P27918
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MABF2127-100UG
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Bab, Ca, RbWB, ELISA, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2127-100UG
      DescriptionAnti-Properdin Antibody, clone 3A3E1
      Alternate Names
      • Complement factor P
      Background InformationProperdin (UniProt: P27918; also known as Complement factor P) is encoded by the CEP (also known as PFC) gene (Gene ID: 5199) in human. (ABF185: Properdin (UniProt P27918; also known as Complement factor P) is encoded by the CFP (also known as PFC, PFD) gene (Gene ID 5199) in human. Properdin is a plasma glycoprotein and the only known positive regulator of the complement cascade. It binds and stabilizes the alternative pathway (AP) C3 convertase C3bBb and may also serve as a platform to form new C3bBb convertases on cell surface. Properdin-mediated AP activation plays an important role in host defense, and properdin deficiency (PFD) increases the risk for Neisseria meningitidis infection in human. Properdin-deficiency exacerbated disease condition in a murine model of factor H- (fH-) related C3 glomerulopathy, but prevented the development of allergen-induced airway inflammation. Human properdin is produced with a signal peptide sequence (a.a. 1-27), which is removed posttranslationally to yield the mature protein (a.a. 28-469) composed entirely of seven TSP type-1 domains (domain 0 through 6; a.a. 28-462). TSP type-1 domains 0 and 6 bind to each other and mediate multimerization.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Applications
      ApplicationThis mouse monoclonal Anti-Properdin, clone 3A3E1 Antibody, Cat. No. MABF2127 is tested for use in Western Blotting, ELISA, Immunohistochemistry (Paraffin)for the detection of Properdin. Inhibitis the functionn of properdin by inhibiting its binding to C3b.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunohistochemistry (Paraffin)
      Application NotesInhibition Analysis: A representative lot detected Properdin in Inhibition applications (Blatt, A.Z., et. al. (2016). J Immunol. 196(11):4671-4680).

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Properdin in human tonsil tissue sections.

      ELISA Analysis: A representative lot detected Properdin in ELISA applications (Blatt, A.Z., et. al. (2016). J Immunol. 196(11):4671-4680).
      Biological Information
      ImmunogenPurified properdin from human plasma.
      Clone3A3E1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3A3E1 specifically detects human properdin.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Baboon
      • Canine
      • Rabbit
      Species Reactivity NoteBaboon, Canine, Rabbit, Human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CEP
      • PFC
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~51 kDa observed; 51.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in purified human properdin.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Properdin in Purified human properdin.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2127-100UG 04054839529511