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MABF2212-100UG Anti-SARS-CoV Nsp8 Antibody, clone 5A10

Anti-SARS-CoV Nsp8, clone 5A10, Cat. No. MABF2212, is a mouse monoclonal antibody that detects Nsp8 of SARS-C0V and is tested for use in Immunofluorescence and Western Blotting.

MABF2212-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2212-100UG
      DescriptionAnti-SARS-CoV Nsp8 Antibody, clone 5A10
      Alternate Names
      • Nonstructural protein NS8
      • ORF8
      Background InformationNonstructural protein NS8 (UniProt: A0A6B9VTP3; also known as ORF8 protein) is encoded by the orf8 (also known as NS8) gene (Gene ID: 43740577) in Sars-CoV-2. Coronaviruses (CoV) represent a diverse family of positive-sense RNA viruses capable of causing respiratory and enteric disease in human and animal hosts. Amongst these the SARS-CoV-2 is the most prominent virus that causes severe respiratory syndrome in human. The nonstructural proteins (nsps) of coronavirus assemble to form the replication transcription complex (RTC) responsible for viral RNA synthesis. In SARS-CoV-2 non-structural protein Nsp8 is a critical component of the RNA replicase and features both well folded secondary structure and disordered segments. It is part of the RNA-dependent RNA polymerase (RdRp) complex and forms a heterotetramer consisting of one molecule of Nsp7, two copies of Nsp8 and one of Nsp12. Nsp8 an Nsp7 are reported to adopt a hollow cylinder-like structure and serve as co-factors for the catalytic Nsp12 that play a role in the stabilization of Nsp12 regions involved in RNA binding and are essential for a highly active Nsp12 polymerase complex. (Ref.: Trevino, MA., et al. (2023). Nucleic Acids Res. 51(18); 10041-10048; Couroble, VV., et al. (2021). J. Am. Soc. Mass Spectrom. 32(7); 1618-1630; Kumar, P., et al. (2007). Virology. 366; 293-303).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-SARS-CoV Nsp8, clone 5A10, Cat. No. MABF2212, is a mouse monoclonal antibody that detects Nsp8 of SARS-C0V and is tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesWestern Blotting Analysis: A representative lot detected Nsp8 in Western Blotting applications (Kumar, P., et al. (2007). Virology. 366(2):293-303).

      Immunofluorescence Analysis: A representative lot detected NSP8 in Immunofluorescence applications (Kumar, P., et al. (2007). Virology. 366(2):293-303).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenGST-tagged recombinant Nonstructural protein NS8 from SARS-CoV-2.
      EpitopeUnknown
      Clone5A10
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 5A10 is a mouse monoclonal antibody that detects Nonstructural protein NS8 of SARS-CoV-2.
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteVirus (SARS-CoV)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • orf8
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~22 kDa observed; 13.83 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant SARS-CoV-2 Nsp8 protein.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected recombinant SARS-CoV-2 Nsp8 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2212-100UG 04065270845221