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ABT13 Anti-Sarcolipin Antibody

Anti-Sarcolipin, Cat. No. ABT13, is a rabbit polyclonal antibody that detects Sarcolipin and is tested for use in Peptide Inhibition Assay and Western Blotting.

Anti-Sarcolipin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: SLN UniProt Number: O00631
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ABT13
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RbWB, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT13
      DescriptionAnti-Sarcolipin Antibody
      Alternate Names
      • Sarcopolin
      Background InformationSarcolipin (UniProt: O00631; also known as SLN) is encoded by the SLN gene (Gene ID: 6588) in human. Sarcolipin is a single-pass membrane protein that contains a cytoplasmic domain (aa 1-7), a transmembrane domain (aa 8-26), and a lumenal domain (aa 27-31). SLN is a regulator of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) activity in muscle and plays a role in regulating muscle thermogenesis and metabolism. It increases the heat generated by SERCA by partially uncoupling Ca2+ re-sequestration from ATP hydrolysis in muscle and contributes to non-shivering thermogenesis. This heat generating process is mechanistically distinct from and unrelated to Ca2+-linked thermogenic processes in skeletal muscle. It acts by reducing the apparent affinity of the ATPase for Ca2+. SLN is also reported to modulate calcium re-uptake during muscle relaxation and play an important role in calcium homeostasis in muscle. Higher levels of SLN have been reported in patients with Duchenne muscular dystrophy and reducing SLN levels improves dystrophic pathology. (Ref.: Campbell, KL., and Dicke, AA (2018). Front. Physiol. 9; Article 714; Voit, A., et al. (2017). Nat. Commun. 8; Article 1068).
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      Control
      • Rabbit quadraceps tissue
      PresentationPurified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Sarcolipin, Cat. No. ABT13, is a rabbit polyclonal antibody that detects Sarcolipin and is tested for use in Peptide Inhibition Assay and Western Blotting.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Assay: Sarcolipin detection in Rabbit Quadricept tissue lysate was completely inhibited by preblocking of this antibody with 1 mg of antigen peptide for 1 hour at 37⁰C .

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human Sarcolipin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Sarcolipin in multiple mammalian species. It targets an epitope within 6 amino acids from the C-terminal lumenal domain.
      Species Reactivity
      • Rabbit
      Species Reactivity NoteRabbit. Predicted to react with Bovine, Human, Mouse, Monkey, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySarcoplasmic reticulum Ca(2+)-ATPases are transmembrane proteins that catalyze the ATP-dependent transport of Ca(2+) from the cytosol into the lumen of the sarcoplasmic reticulum in muscle cells. This gene encodes a small proteolipid that regulates several sarcoplasmic reticulum Ca(2+)-ATPases. The transmembrane protein interacts with Ca(2+)-ATPases and reduces the accumulation of Ca(2+) in the sarcoplasmic reticulum without affecting the rate of ATP hydrolysis.
      Gene Symbol
      • SLN
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummarySUBUNIT STRUCTURE: Associated with calcium ATPase SERCA1.

      SUBCELLULAR LOCATION: Sarcoplasmic reticulum membrane; Single-pass membrane protein.

      SEQUENCE SIMILARITIES: Belongs to the sarcolipin family.
      Molecular Weight~6 kDa observed; 3.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rabbit Quadriceps muscle tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Sarcolipin in Rabbit Quadriceps muscle tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABT13 04053252644450

      Documentation

      Anti-Sarcolipin Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Sarcolipin Antibody Certificates of Analysis

      TitleLot Number
      Anti-Sarcolipin - 2406996 2406996
      Anti-Sarcolipin - 2449231 2449231
      Anti-Sarcolipin - 2159658 2159658
      Anti-Sarcolipin - 2193358 2193358
      Anti-Sarcolipin - 3013471 3013471
      Anti-Sarcolipin - 3143173 3143173
      Anti-Sarcolipin - 3537160 3537160
      Anti-Sarcolipin - 3598057 3598057
      Anti-Sarcolipin - 3684752 3684752
      Anti-Sarcolipin - 3768101 3768101
      Show more

      References

      Reference overviewPub Med ID
      The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity.
      Butler, J; Smyth, N; Broadbridge, R; Council, CE; Lee, AG; Stocker, CJ; Hislop, DC; Arch, JR; Cawthorne, MA; Malcolm East, J
      Archives of biochemistry and biophysics  569  26-31  2015

      Show Abstract       		25660043 25660043

      Brochure

      Title
      New Products: Volume 3, 2012

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies