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MABN1534 Anti-TREM-2 Antibody, clone 10H4.2

Anti-TREM-2 Antibody, clone 10H4.2 is an antibody against TREM-2 for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-TREM-2 Antibody, clone 10H4.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neuroinflammation & Pain Gene Symbol: TREM2 UniProt Number: Q9NZC2
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MABN1534
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1534
      DescriptionAnti-TREM-2 Antibody, clone 10H4.2
      Alternate Names
      • Triggering receptor expressed on myeloid cells 2
      • TREM-2
      • Triggering receptor expressed on monocytes 2
      Background InformationTriggering receptor expressed on myeloid cells 2 (UniProt Q9NZC2; also known as TREM-2, Triggering receptor expressed on monocytes 2) is encoded by the TREM2 gene (Gene ID 54209) in human. TREM2 is an Ig-like V-type receptor expressed by populations of myeloid cells in the central nervous system and periphery. TREM2 is highly expressed in microglia, where it, along with its adaptor protein DAP12, regulates inflammatory cytokine release and phagocytosis of apoptotic neurons. Apolipoprotein E (apoE) is reported to function as a high-affinity TREM2 ligand. ApoE binding enhances the phagocytosis of apoptotic N2a cells by primary microglia cells, and greatly reduced ApoE binding is seen with AD-associated TREM2 R47H mutatant. TREM2 is produced with a signal peptide sequence (a.a. 1-18), the removal of which yields the mature single-transmembrane (a.a. 175-195) protein with a large extracellular region (a.a. 19-174) that contains the V-type Ig-like domain (a.a. 29-112) and a cytoplasmic tail (a.a. 196-230). The short cytoplasmic tail contains no signaling motifs and relies on its association with the immunoreceptor tyrosine-based activation motif- (ITAM-) containing TYROBP for signal transduction. TREM2 gene mutations and polymorphisms are linked to several neurological disorders, including Alzheimer's disease (AD), frontotemporal dementia (FTD), Parkinson's disease (PD), and Nasu-Hakola disease (a.k.a. PLOSL or polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-TREM-2 Antibody, clone 10H4.2 is an antibody against TREM-2 for use in Western Blotting, Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TREM-2 in human bone marrow, spleen and cerebellum tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a cytoplasmic domain sequence of human TREM-2.
      EpitopeCytoplasmic domain.
      Clone10H4.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 10H4.2 recognizes a cytoplasmic domain epitope that is not present in the spliced isoforms 2&3 reported by UniProt (Q9NZC2).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TREM2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~30 kDa oberved. Target band size appears larger than the calculated moleuclar weights of 25.45 kDa (pro-form) and 23.45 kDa (mature form) due to glycosylation. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human spleen tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected TREM-2 in 10 µg of human spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1534 04055977350449