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MABE432 Anti-TRIM33 Antibody, clone 3B5.1

This Anti-TRIM33 Antibody, clone 3B5.1 is validated for use in Western Blotting and Immunocytochemistry for the detection of TRIM33.

Anti-TRIM33 Antibody, clone 3B5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: TRIM33, KIAA1113, RFG7, TIF1G UniProt Number: Q9UPN9
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MABE432
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE432
      DescriptionAnti-TRIM33 Antibody, clone 3B5.1
      Alternate Names
      • E3 ubiquitin-protein ligase TRIM33
      • Ectodermin homolog
      • RET-fused gene 7 protein
      • Protein Rfg7
      • Transcription intermediary factor 1-gamma
      • TIF1-gamma
      • Tripartite motif-containing protein 33
      Background InformationTRIM33, also known as E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, RET-fused gene 7 protein, Rfg7, Transcription intermediary factor 1-gamma, TIF1-gamma, or Tripartite motif-containing protein 33, and encoded by the gene TRIM33/KIAA1113/RFG7/TIF1G, is an E3 ubiquitin protein ligase. Among its targets is SMAD4 for which ubiquitination facilitates its nuclear exclusion and degradation. SMAD3 is also a target for ubiquitination, and together, TRIM33's association promotes erythroid differentiation of hematopoietic stem cells. Finally mono-ubiquitination of various targets by TRIM33 affects their ability to form complexes which often hampers their activity and thus regulates their subsequent actions. Such is the case of SMAD4 and its interaction with TGF-beta/BMP signaling. Monoubiquitination of SMAD4 by TRIM33 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. TRIM33 is localized in nucleus in nuclear dots or bodies and is expressed in most tissues and stem cells during development as well as in the adult in multiple tissues.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-TRIM33 Antibody, clone 3B5.1 is validated for use in Western Blotting and Immunocytochemistry for the detection of TRIM33.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected TRIM33 in H9 stem cells.
      Alexa Fluor® is a registered trademark of Life Technologies®.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human TRIM33.
      Clone3B5.1
      Concentration1 mg/mL
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TRIM33
      • KIAA1113
      • RFG7
      • TIF1G
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~123 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected TRIM33 in 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE432 04053252978777