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ABS2230-100UL Anti-USP46

Anti-USP46, Cat. No. ABS2230, is a rabbit polyclonal antibody that detects USP46 and is tested for use in Western Blotting, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation, and Immunohistochemistry (Paraffin).

ABS2230-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS2230-100UL
      DescriptionAnti-USP46
      Alternate Names
      • Ubiquitin carboxyl-terminal hydrolase 46
      • EC:3.4.19.12
      • Deubiquitinating enzyme 46
      • Ubiquitin thioesterase 46
      • Ubiquitin-specific-processing protease 46
      Background InformationUbiquitin carboxyl-terminal hydrolase 46 (UniProt: P62068; also known as Deubiquitinating enzyme 46, Ubiquitin thioesterase 46, Ubiquitin-specific-processing protease 46, EC:3.4.19.12) is encoded by the USP46 gene (Gene ID: 64854) in human. USP46 gene encodes cysteine protease belonging to the ubiquitin-specific protease (USP) family of proteins. The protein contains a large USP domain (aa 35-365) and functions as a deubiquitinating enzyme capable of modifying cellular proteins. Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 cleaves ubiquitin from a ubiquitin-β-galactosidase fusion protein. These proteins regulate ubiquitin-mediated pathways that are involved in normal and pathological functions. It is also involved in the regulation of GABA action. Studies show that USP46, along with USP12, deubiquitinates both histone H2A and H2B. USP46 is predominantly localized in the cytoplasm. It is broadly expressed in multiple tissues, with high expression in heart muscle and CNS. Mutations or dysregulation of USP46 have been implicated in various diseases, particularly neuropsychiatric and neurological disorders. Additionally, USP46 may play a role in cancer, where its function in regulating protein stability and signaling pathways can contribute to tumor progression and resistance to therapy.(Ref.: Joo, et al. (2011). JBC 286(9); 7190-7201; Huo, Y., et al. (2015). J. Neurochem. 134(6); 1067-1080; Spencer, Z., et al. (2023). Nat Commun. 5;14(1); 6174).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antiserum without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-USP46, Cat. No. ABS2230, is a rabbit polyclonal antibody that detects USP46 and is tested for use in Western Blotting, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation, and Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Chromatin Immunoprecipitation (ChIP)
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected USP46 in Western Blotting applications (Joo, et al. (2011) JBC 286(9); 7190-7201).

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected USP46 in Human kidney tissue sections.

      Immunoprecipitation Analysis: A representative lot detected USP46 in Immunoprecipitation applications (Joo, et al. (2011) JBC 286(9); 7190-7201).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected USP46 in Chromatin Immunoprecipitation applications (Joo, et al. (2011) JBC 286(9); 7190-7201).

      Immunocytochemistry Analysis: A representative lot detected USP46 in Immunocytochemistry applications (Huo Y. (2015) J. Neurochem. 134(6); 1067-1080).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenFLAG-tagged, full length recombinant protein of USP46.
      EpitopeUnknown
      ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects USP46.
      IsotypeIgGκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • USP46
      Purification MethodUnpurified
      UniProt Number
      Molecular WeightTarget molecular weight ~42 kDa observed; 42.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysates from USP46 (KO) 293T cells and wild type 293T cells.

      Western Blotting Analysis (WB): A 1:1000 dilution of this antibody detected USP46 in lysate from wild type 293T cells, but not in USP46 (KO) 293T cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS2230-100UL 04065272163651