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MABT522 Anti-alpha-Tubulin Antibody, clone RM113

This Anti-alpha-Tubulin Antibody, clone RM113 is validated for use in Western Blotting, Immunocytochemistry for the detection of alpha-Tubulin.

Anti-alpha-Tubulin Antibody, clone RM113 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: TUBA1B UniProt Number: P68363
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MABT522
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT522
      DescriptionAnti-alpha-Tubulin Antibody, clone RM113
      Alternate Names
      • Tubulin alpha-1B chain
      • Alpha-tubulin ubiquitous
      • Tubulin K-alpha-1
      • Tubulin alpha-ubiquitous chain
      • alpha-Tubulin
      Background InformationMicrotubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
      References
      Product Information
      FormatPurified
      PresentationRabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-alpha-Tubulin Antibody, clone RM113 is validated for use in Western Blotting, Immunocytochemistry for the detection of alpha-Tubulin.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected alpha-Tubulin in A431 cells.
      Biological Information
      ImmunogenA peptide corresponding to the C-terminus of α-Tubulin.
      EpitopeC-terminus
      CloneRM113
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • TUBA1B
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~53 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected alpha-Tubulin in A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT522 04055977181548

      Documentation

      Anti-alpha-Tubulin Antibody, clone RM113 Certificates of Analysis

      TitleLot Number
      Anti-alpha-Tubulin, clone RM113 - VP1903091 VP1903091
      Anti-alpha-Tubulin, clone RM113 - VP2202183 VP2202183
      Anti-alpha-Tubulin, clone RM113 - VP2310033 VP2310033
      Anti-alpha-Tubulin, clone RM113 - VP2409055 VP2409055
      Anti-alpha-Tubulin, clone RM113 -QVP1503208 QVP1503208
      Anti-alpha-Tubulin, clone RM113 Monoclonal Antibody VP1706196

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.