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AB15708-25UG Anti- beta III Tubulin

Anti-β III Tubulin, Cat. No. AB15708, is a rabbit polyclonal antibody that detects β III Tubulin and is tested for use in Immunocytochemistry and Western Blotting.

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AB15708-25UG
25 μg  
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      Description
      Catalogue NumberAB15708-25UG
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti- beta III Tubulin
      Alternate Names
      • Tubulin beta-3 chain
      • Tubulin beta-4 chain
      • Tubulin beta-III
      • beta-Tubulin 3
      Background InformationTubulin beta-3 chain (UniProt: Q13509; also known as Tubulin beta-4 chain, Tubulin beta-III, beta-Tubulin 3) is encoded by the TUBB3 (also known as TUBB4) gene (Gene ID: 10381) in human. Tubulin is one of several members of a small family of globular proteins, which polymerize to form microtubules. The most common members of the tubulin family are -tubulin and β-tubulin that assemble onto microtubules forming large polymers. Tubulin binds two moles of GTP, one at an exchangeable site on the β-chain and one at a non-exchangeable site on the -chain. GTP-binding site of β-tubulin III is localized to amino acids 140-146. β-tubulin III is a unique microtubule subunit that is expressed exclusively in neurons, but its higher expression is also observed in most cancerous tissues. Due to it is unique expression pattern it is often utilized as a marker to identify neuronal cells from other brain cells such as glia. It plays a critical role in proper axon guidance and maintenance. It undergoes phosphorylation on serine 172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase and this phosphorylation inhibits tubulin incorporation into microtubules. Increased expression of b-tubulin III been shown to correlate with resistance to anti-microtubule agents. Mutations in TUBB3 gene have been linked to congenital fibrosis of extraocular muscles that causes ocular motility disorder affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. Some mutations are also known to cause cortical dysplasia that results in aberrant neuronal migration and disturbed axonal guidance. (Ref.: Poirier, K.., et al. (2010). Hum. Mol. Gen. 19(22); 4462-4473; Tischfield, MA., et al. (2010). Cell. 140(1); 74-87; Katsetos, CD., et al. (2003). J. Child. Neurol. 18(12); 851-866).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-β III Tubulin, Cat. No. AB15708, is a rabbit polyclonal antibody that detects β III Tubulin and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected β III Tubulin in Rat Brain tissue lysate.

      Immunocytochemistry Analysis: A 1:800 dilution from a representative lot detected β III Tubulin in Neuro2a cells.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids from the C-terminal region of Human β III Tubulin.
      EpitopeC-terminal
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects β III tubulin. It targets an epitope within 10 amino acids from the C-terminal region.
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteMouse, Rat. Predicted to react with Monkey, Bovine, Human based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TUBB3
      • TUBB4
      • MC1R
      • beta-4
      Purification MethodAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
      SIZE: 450 amino acids; 50433 Da
      SUBUNIT: Dimer of alpha and beta chains.
      DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
      SIMILARITY: Belongs to the tubulin family.
      Molecular Weight~50 kDa observed; 50.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse brain tissue lysate.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected β III Tubulin in Mouse Brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB15708-25UG 04054839342653

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