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MABF2057-25UG Anti-minicore HCV Antibody, clone Neo4

Anti-minicore HCV, clone Neo4, Cat. No. MABF2057, is a mouse monoclonal antibody that detects HCV core and minicore proteins in HCV infected cells and has been tested for use in Immunofluorescence and Western Blotting.

Anti-minicore HCV Antibody, clone Neo4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology UniProt Number: Q9WMX2
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MABF2057-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VirusIF, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2057-25UG
      DescriptionAnti-minicore HCV Antibody, clone Neo4
      Alternate Names
      • HCV
      • Hepatitis C virus minocores
      Background InformationHepatitis C virus (HCV) is a small (~55 65 nm) in size), enveloped, positive-sense single-stranded RNA virus that is a causative factor for hepatitis C and hepatocellular carcinoma. HCV expresses several proteins that promote viral replication. Genome polyprotein of HCV is a core protein that packages viral RNA to form a viral nucleocapsid, and promotes virion budding. It also modulates viral translation initiation by interacting with HCV IRES and 40S ribosomal subunit. It prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways and by inducing human STAT1 degradation. Envelope (E1 and E2) glycoproteins of HCV form a heterodimer, which is involved in virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. P7 protein, a heptameric ion channel protein, is essential for infectivity. HCV also contains NS2-3, which acts as a cysteine protease responsible for the autocatalytic cleavage of NS2-NS3. It undergoes self-inactivation following maturation. NS3 protein of HCV displays serine protease, NTPase, and RNA helicase activities. NS4B protein is shown to induce a specific membrane alteration that serves as a scaffold for the virus replication complex. NS5A protein is a component of the replication complex and is involved in RNA-binding. It is reported to mediate interferon resistance by interacting with and inhibiting human EIF2AK2/PKR. HCV also expresses proteins that are derived from the core gene and are known as minocores. These minicores lack the N-terminus, but contain the C-terminal portion of the mature p21 nucleocapsid protein. They are associated with higher risk of hepatocellular carcinoma, insulin resistance, and failure of interferon-based therapy.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-minicore HCV, clone Neo4, Cat. No. MABF2057, is a mouse monoclonal antibody that detects HCV core and minicore proteins in HCV infected cells and has been tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected minicore HCV in Western Blotting applications (Eng, F.J., et. al. (2017). Hepatol Commun. 2(1):21-28).

      Immunofluorescence Analysis: A representative lot detected minicore HCV in Immunofluorescence applications (Eng, F.J., et. al. (2017). Hepatol Commun. 2(1):21-28).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of Hepatitis C virus (HCV).
      CloneNeo4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone Neo4 detects HCV core and minicore proteins in cells infected with HCV virus.
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteVirus.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~ 8 and 21 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in extracts of HEK293T cells transfected with plasmids expressing only HCV p21 core and 91 minicore proteins.

      Western Blotting Analysis: 4 ug/mL of this antibody detected minicore HCV in extracts of HEK293T cells transfected with plasmids expressing only HCV p21 core and 91 minicore proteins.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2057-25UG 04054839516856