Millipore Sigma Vibrant Logo

ABE1464-25UG Anti-monomethyl Histone H2B (Pro1)

Anti-monomethyl Histone H2B (Pro1) Antibody, Cat. No. ABE1464, is a highly specific rabbit polyclonal antibody that targets Histone H2B monomethylated on proline 1 and has been tested for use in Western Blotting and Peptide Inhibition Assay.

Anti-monomethyl Histone H2B (Pro1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: HIST1H2BC, H2BFL, HIST1H2BE, H2BFH, HIST1H2BF, H2BFG, HIST1H2BG, H2BFA, HIST1H2BI, H2BFK UniProt Number: P62807
View Products on Sigmaaldrich.com
ABE1464-25UG
25 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Fulfillment and delivery delayed Fulfillment and delivery delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIARbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1464-25UG
      DescriptionAnti-monomethyl Histone H2B (Pro1)
      Alternate Names
      • Histone H2B type 1-C/E/F/G/I
      • Histone H2B.1 A
      • Histone H2B.a
      • H2B/a
      • Histone H2B.g
      • H2B/g
      • Histone H2B.h
      • H2B/h
      • Histone H2B.k
      • H2B/k
      • Histone H2B.l
      • H2B/l
      Background InformationHistone H2B type 1-C/E/F/G/I (UniProt: P62807; also known as Histone H2B.1 A, Histone H2B.a, H2B/a, Histone H2B.g, H2B/g, Histone H2B.h, H2B/h, Histone H2B.k, H2B/k, Histone H2B.l, H2B/l) is encoded by the HIST1H2BC (also known as H2BFL, HIST1H2BE, H2BFH, HIST1H2BF, H2BFG, HIST1H2BG, H2BFA, HIST1H2BI, H2BFK) gene (Gene ID: 3017, 9339, 8343, 8344, 8346, 8347) in human. Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. They undergo a number of post-translational modifications (PTM) at their N- and C-terminal tails which can influence chromatin structure and stability; and consequently, transcription, and DNA replication and repair. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H2B is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone H2B can be monoubiquitinated on Lysine123 by RAD6/UBC2-BRE1 complex to form H2BK123Ub1 complex that gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. H2BK123ub1 is reported to modulates the formation of double-strand breaks during meiosis and is a required for DNA-damage checkpoint activation. Methylation of N-terminus Proline 1 of histone H2B (Pme) by NTMT (CG1675) is one of the rare modification that has been addressed. Heat shock or arsenite treatment is shown to increase methylation on proline 1 in Drosophila cells. (Ref.: Villar-Garea, A., et al. (2012). Nucl. Acid Res. 40(4); 1536 1549; Desrosiers, R., and Tanguay, RM. (1998). J. Biol. Chem. 263(10):4686-4692).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-monomethyl Histone H2B (Pro1) Antibody, Cat. No. ABE1464, is a highly specific rabbit polyclonal antibody that targets Histone H2B monomethylated on proline 1 and has been tested for use in Western Blotting and Peptide Inhibition Assay.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Analysis: A 1:500 dilution from a representative lot was used with HeLa acid extract for peptide block analysis.



      Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides were probed with Anti-monomethyl Histone H2B (Pro1) (20 ng (A), 10 ng (B), 5 ng (C), 2.5 ng (D) dilutions).



      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected monomethyl Histone H2B (Pro1) in HeLa, A431, HUVEC, and NIH3T3 cell lines.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the first 9 amino acids (methiionine removed) from the N-terminus region of human Histone H2B1C with methylated proline.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detect Histone H2B monomethylated on proline 1.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H2BC
      • H2BFL
      • HIST1H2BE
      • H2BFH
      • HIST1H2BF
      • H2BFG
      • HIST1H2BG
      • H2BFA
      • HIST1H2BI
      • H2BFK
      Modifications
      • Methylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~16 kDa observed; 13.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cells acid extract.

      Western Blotting Analysis: 12 µg/mL of this antibody detected monomethyl Histone H2B (Pro1) in HeLa acid extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1464-25UG 04054839499524