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MABS2309-25UL Anti-p66shc Antibody, clone BG8

Anti-p66shc, clone BG8, Cat. No. MABS2309, is a mouse monoclonal antibody that detects SHC-transforming protein 1 (p66shc) and is tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.

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MABS2309-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2309-25UL
      DescriptionAnti-p66shc Antibody, clone BG8
      Alternate Names
      • SHC-transforming protein 1
      • SHC-transforming protein 3
      • SHC-transforming protein A
      • Src homology 2 domain-containing-transforming protein C1
      • SH2 domain protein C1
      Background InformationSHC-transforming protein 1 (UniProt: P29353; also known as SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1, SH2 domain protein C1) is encoded by the SHC1 (also known as SHC, SHCA) gene (Gene ID: 6464) in human. p66Shc is a splice variant of p52Shc/p46Shc with a typical domain organization that is shared by all members of the Shc family of adaptor proteins. It has a phosphotyrosine binding domain, a collagen homology 1 region, and an SH2 domain. p66Shc is shown to be a downstream target of p53 and activated p53 induces its up-regulation by increasing its stability. p66Shc is suggested to be a genetic determinant of aging in mammals as its homozygous deletion is reported to delay senescence and prolong life span in mice. It regulates the mitochondrial pathway of apoptosis by inducing mitochondrial damage after dissociation from an inhibitory protein complex. Under conditions of excessive reactive oxygen species (ROS), p66Shc is phosphorylated on serine 36 by protein kinase C and is targeted to the mitochondrial intermembrane space where it serves as a redox enzyme by oxidizing cytochrome c and reducing oxygen to form ROS. In the absence of p66Shc, release of cytochrome c from mitochondria is impaired and apoptosome can only be activated if purified cytochrome c is supplied. p66Shc induces the collapse of the mitochondrial trans-membrane potential after oxidative stress. p66Shc is also shown to have a neuroprotective role in a murine model of multiple sclerosis. p66Shc knockout (p66-KO) mice develop typical signs of experimental autoimmune encephalomyelitis (EAE). (Ref.: Su, KG., et al. (2012). Eur. J. Neurosci. 35; 562-571; Orsini, F., et al. (2004). J. Biol. Chem. 279(24); 25689-25695).
      References
      Product Information
      FormatPurified
      Control
      • MilliporeSigma offers a comprehensive range of trusted and highly cited primary and secondary antibodies for your research needs. Explore our 94
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      • Cell structure
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      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-p66shc, clone BG8, Cat. No. MABS2309, is a mouse monoclonal antibody that detects SHC-transforming protein 1 (p66shc) and is tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunofluorescence
      Application NotesTested applications

      Immunofluorescence Analysis: A representative lot detected p66shc 1 in Immunofluorescence application (Orsini, F., et al. (2004). J Biol Chem. 279(24): 25689-95).

      Immunocytochemistry Analysis: A representative lot detected p66shc in Immunocytochemistry application (Orsini, F., et al. (2004). J Biol Chem. 279(24): 25689-95).

      Western Blotting Analysis: A representative lot detected p66shc in Western Blotting application (Orsini, F., et al. (2004). J Biol Chem. 279(24): 25689-95); Su, K.G., et al. (2012). Eur J Neurosci. 35(4):562-71).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to the first 110 amino acids from the N-terminal region of human SHC-transforming protein 1 (p66Shc).
      EpitopeN-terminal
      CloneBG8
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone BG8 is a mouse monoclonal antibody that detects p66Shc. It was generated in p66Shc-/- mice. It targets an epitope within the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Human based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SHC1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~62 kDa observed; 62.82 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH3T3 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected SHC-transforming protein 1 in NIH3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2309-25UL 04065269048305