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ABS1849 Anti-phospho-Rsk1 (Thr359/Ser363) Antibody

Anti-Rsk1 Antibody is an antibody against phospho-Rsk1 (Thr359/Ser363) for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Peptide Inhibition Assay.

Anti-phospho-Rsk1 (Thr359/Ser363) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Kinases & Phosphatases Gene Symbol: RPS6KA1, MAPKAPK1A, RSK1 UniProt Number: Q15418
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ABS1849
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, IHC, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1849
      DescriptionAnti-phospho-Rsk1 (Thr359/Ser363) Antibody
      Alternate Names
      • Ribosomal protein S6 kinase alpha-1, Thr359/Ser363 phosphorylated
      • 90 kDa ribosomal protein S6 kinase 1, Thr359/Ser363 phosphorylated
      • MAP kinase-activated protein kinase 1a, Thr359/Ser363 phosphorylated
      • MAPK-activated protein kinase 1a, Thr359/Ser363 phosphorylated
      • MAPKAP kinase 1a, Thr359/Ser363 phosphorylated
      • MAPKAPK-1a, Thr359/Ser363 phosphorylated
      • p90-RSK 1, Thr359/Ser363 phosphorylated
      • p90RSK1, Thr359/Ser363 phosphorylated
      • p90S6K, Thr359/Ser363 phosphorylated
      • Ribosomal S6 kinase 1, Thr359/Ser363 phosphorylated
      • RSK-1, Thr359/Ser363 phosphorylated
      • S6K-alpha-1, Thr359/Ser363 phosphorylated
      Background InformationRibosomal protein S6 kinase alpha-1 (EC 2.7.11.1; UniProt Q15418; also known as 90 kDa ribosomal protein S6 kinase 1, MAP kinase-activated protein kinase 1a, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, p90-RSK 1, p90RSK1, p90S6K, Ribosomal S6 kinase 1, RSK-1, S6K-alpha-1) is encoded by the RPS6KA1 (also known as MAPKAPK1A, RSK1) gene (Gene ID 6195) in human. Ribosomal s6 kinases (RSKs) are serine/threonine kinases that play a role in mediating Mek1/2-Erk1/2 mitogen-activated protein (MAP) kinase pathway signaling events. There exist two types of RSKs, p70rsk (a.k.a. S6 Kinase or S6-H1 kinase) and p90rsk (a.k.a. MAPK-activated protein kinase). Six p90rsk-coding genes have been identified in human, RPS6KA1 (MAPKAPK1A, RSK1), RPS6KA2 (MAPKAPK1C, RSK3), RPS6KA3 (MAPKAPK1B, RSK2), RPS6KA4 (MSK2), RPS6KA5 (MSK1), and RPS6KA6 (RSK4). Erk1/2 activates p90RSK1 by sequential phosphorylation events, where it first phosphorylates Rsk1 C-terminal kinase domain (CTKD) Thr573, leading to subsequent Ser380 phosphorylation in the N-terminal AGC kinase-type domain. Alternatively, Ser380 can be phosphorylated directly by MK2/3 independent of CTKD.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Rsk1 Antibody is an antibody against phospho-Rsk1 (Thr359/Ser363) for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Peptide Inhibition Assay.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry
      • Peptide Inhibition Assay
      Application NotesImmunoprecipitation Analysis: 4.5 µg from a representative lot immunoprecipitated Thr359/Ser363-phosphorylated Rsk1 from 500 µg of lysate from EGF-treated A431 cells.
      Immunohistochemistry Analysis: A 1:250-1000 dilution from a representative lot detected Rsk1 Thr359/Ser363 phosphorylation in human cervical cancer tissue, normal kidney, liver, and small intestine tissue sections.
      Peptide Inhibition Assay: Preincubation with the immunogen peptide, but not the corresponding non-phosphorylated control peptide, prevented the polyclonal antibody from detecting the target Rsk1 phosphorylation band.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a human Rsk1-derived sequence containing phosphorylated Thr359 and Ser363 residues.
      Epitope pThr359/Ser363.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificitySpecificity of this polyclonal antibody was verified by immunogen peptide blocking in Western blotting analysis of EGF-induced target phosphorylation. The target phosphorylation sites Thr359/Ser363 are numbered based on human Rsk1 spliced isoform 1 (UniProt Q15418-1). Equivalent sites in spliced isoform 2, 3, and 4 are Thr368/Ser372 (UniProt Q15418-2), Thr267/Ser271 (UniProt Q15418-3), and Thr343/Ser347 (UniProt Q15418-4), respectively.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Xenopus, Bovine, Porcine, Chicken based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RPS6KA1
      • MAPKAPK1A
      • RSK1
      Modifications
      • Phosphorylation
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~75 kDa observed. 82.72 kDa (human isoform 1), 83.93 kDa (human isoform 2), 72.70 kDa (human isoform 3), and 81.15 kDa (human isoform 4) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: 0.1 µg/mL of this antibody detected enhanced Rsk1 Thr359/Ser363 phosphorylation in 10 µg of lysate from EGF-treated A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1849 04055977349412

      Documentation

      Anti-phospho-Rsk1 (Thr359/Ser363) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-Rsk1 (Thr359/Ser363) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-Rsk1 (Thr359/Ser363) - 3195391 3195391
      Anti-phospho-Rsk1 (Thr359/Ser363) - 3425387 3425387
      Anti-phospho-Rsk1 (Thr359/Ser363) - 3500935 3500935
      Anti-phospho-Rsk1 (Thr359/Ser363) - 3890508 3890508
      Anti-phospho-Rsk1 (Thr359/Ser363) -Q2676855 Q2676855
      Anti-phospho-Rsk1 (Thr359/Ser363) Polyclonal Antibody 3118632