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HTS090M ChemiScreen™ Recombinant Human OT Oxytocin Receptor Membrane Preparation

Human OT GPCR membrane prepation for Radioligand binding Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Uni Prot Number: P30559 Entrez Gene Number: NM_000916.3
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HTS090M
200 units  
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      Overview

      Replacement Information

      Key Spec Table

      SpeciesHost CellsProtein Target
      Human Chem-1OT
      Description
      Catalogue NumberHTS090M
      Brand Family Chemicon®
      Trade Name
      • ChemiScreen
      • Chemicon
      DescriptionChemiScreen™ Recombinant Human OT Oxytocin Receptor Membrane Preparation
      OverviewHuman Oxytocin Receptor
      Background InformationOxytocin is a cyclic 9 amino acid peptide that differs from vasopressin in 2 amino acids. Despite the close similarities in sequence, oxytocin and vasopressin have different biological activities and bind to distinct G protein-coupled receptors. The oxytocin receptor, OT, couples primarily to Gq/11 to mobilize intracellular calcium. In female reproduction, oxytocin promotes uterine contraction and lactation; oxytocin is the most commonly used drug for induction of labor, whereas an oxytocin antagonist, atosiban, is under investigation to suppress preterm labor. Oxytocin/OT interaction in the CNS also plays an important role in stress, male and female sexual response, and sociality (Gimpl and Fahrenholz, 2001). Chemicon's oxytocin membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of oxytocin receptor interactions with oxytocin. The membrane preparations exhibit a Kd of 0.53nM for [125I]-OVTA. With 5.0 ug/well Oxytocin Membrane Prep and 0.5 nM [125I]-OVTA, a greater than 6-fold signal-to-background ratio was obtained.
      References
      Product Information
      FormatMembranes
      PresentationOne package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 6-fold signal:background with 125I-labeled OVTA at 0.5 nM.

      Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
      Packaging method: Membranes protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
      Quality LevelMQ100
      Applications
      ApplicationHuman OT GPCR membrane prepation for Radioligand binding Assays.
      Key Applications
      • Radioligand binding assay
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the G-protein coupled receptor family and acts as a receptor for oxytocin. Its activity is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. The oxytocin-oxytocin receptor system plays an important role in the uterus during parturition.
      Gene Symbol
      • OXTR
      • OT-R
      GPCR ClassA
      Host CellsChem-1
      Protein TargetOT
      Target Sub-FamilyOxytocin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P30559 # Receptor for oxytocin. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol- calcium second messenger system.
      SIZE: 389 amino acids; 42772 Da
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      SIMILARITY: SwissProt: P30559 ## Belongs to the G-protein coupled receptor 1 family.
      Incubation ConditionsMembranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

      Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
      Radioligand: [125I] OVTA (Perkin Elmer # NEX254)
      Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Signal:background and specific binding values obtained in a competition binding assay with varying amounts of Oxytocin Receptor membrane prep:
      Product Usage Statements
      5 µg/well 10 µg/well
      Signal:Background 9 9
      Specific Binding (cpm) 21871 24926


      SPECIFICATIONS: 1 unit = 5 µg membrane preparation
      Bmax: 4.5 pmol/mg
      Kd: 0.53 nM Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
      Packaging Information
      Material Size200 units
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      HTS090M 04053252475900

      Documentation

      ChemiScreen™ Recombinant Human OT Oxytocin Receptor Membrane Preparation SDS

      Title

      Safety Data Sheet (SDS) 

      ChemiScreen™ Recombinant Human OT Oxytocin Receptor Membrane Preparation Certificates of Analysis

      TitleLot Number
      CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN OT OXYTOCIN RECEPTOR - 2135594 2135594
      CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN OT OXYTOCIN RECEPTOR - 2210074 2210074

      References

      Reference overviewPub Med ID
      The oxytocin receptor system: structure, function, and regulation
      Gimpl, G and Fahrenholz, F
      Physiol Rev, 81:629-683 (2001)  2001

      		11274341 11274341

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