Millipore Sigma Vibrant Logo

115937 Fuchsin (C.I. 42510)

View Products on Sigmaaldrich.com
115937
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
1159370025
Retrieving availability...
Fulfillment and delivery delayed
Fulfillment and delivery delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      25 g
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      Request Pricing
      Description
      Catalogue Number115937
      DescriptionFuchsin (C.I. 42510)
      OverviewThis staining dye “Fuchsin (C.I. 42510) - for microscopy Certistain®” is used for staining of acid-fast bacteria (AFB) according to Kinyoun and serves the purpose of the bacteriological and histological investigation of sample material of human origin, for example smears of enriched bacterial cultures or histological sections of e. g. the lung.
      The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. As a measure to enhance the absorption of the fuchsin dye and thus the formation of the mycolate-fuchsin complex in the cell wall, the carbolfuchsin solution applied to the specimen is normally heated to evaporation point. If the Kinyoun carbolfuchsin solution is used, the heating of the solution is dispensable. Consequently, the release of hazardous phenolic vapors will be avoided.
      Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In the present operating instructions is counterstained accordance with methylene blue.
      Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      References
      Product Information
      Hill FormulaC₂₀H₂₀ClN₃
      Molar Mass337.85 g/mol
      HS Code3212 90 00
      Structure formula ImageStructure formula Image
      Quality LevelMQ400
      Applications
      Applicationfor microscopy Certistain®
      Biological Information
      Physicochemical Information
      Melting Point235 °C (decomposition)
      pH value5 - 6 (1 g/l, H₂O, 25 °C)
      Bulk density500 kg/m3
      Solubility4 g/l
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Statement(s)H315: Causes skin irritation.
      H319: Causes serious eye irritation.
      H350: May cause cancer.
      H410: Very toxic to aquatic life with long lasting effects.
      Precautionary Statement(s)P202: Do not handle until all safety precautions have been read and understood.
      P264: Wash skin thoroughly after handling.
      P273: Avoid release to the environment.
      P302 + P352: IF ON SKIN: Wash with plenty of water.
      P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
      P308 + P313: IF exposed or concerned: Get medical advice/ attention.
      Signal WordDanger
      RTECSCX9850000
      Storage class6.1C Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
      WGKWGK 3 highly hazardous to water
      Disposal9
      Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
      Safety Information
      Categories of dangercarcinogenic
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +2°C to +30°C.
      Packaging Information
      Transport Information
      Declaration (railroad and road) ADR, RIDUN 3077 , 9, III
      Declaration (transport by air) IATA-DGRUN 3077 , 9, III
      Declaration (transport by sea) IMDG-CodeUN 3077 , 9, III, Marine Pollutant: P
      Supplemental Information
      Specifications
      Dye content (spectrophotometrically)≥ 80 %
      Identity (UV/VIS-Spectrum)passes test
      Absorption maximum λ max (ethanol 50 %)549 - 552 nm
      Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %)2150 - 2710
      TLC-Testpasses test
      Loss on drying (110 °C)≤ 15 %
      Suitability for microscopypasses test
      Global Trade Item Number
      Catalogue Number GTIN
      1159370025 04022536162362

      Related Products & Applications

      Accessories (Secondary)

      Catalogue Number Description  
      100983 Ethanol Show Pricing & Availability
      115943 Methylene blue (C.I.52015) Show Pricing & Availability

      Related Products By: Application Facete

      Related Products By: Brand Facete

      Categories

      IVD/OEM Materials & Reagents > Microscopy > Dyes for Microscopy > Certistain® Dyes