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13-118 GST-NFκB p50 subunit (δ434-969)

N-terminal GST-tagged, recombinant human NFkB, p50 subunit (D434-969), expressed in E. coli. Purified using glutathione agarose chromatography.

GST-NFκB p50 subunit (δ434-969) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: P19838 Entrez Gene Number: NM_003998.2
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13-118
50 µg  
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      Overview

      Replacement Information
      Description
      Catalogue Number13-118
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionGST-NFκB p50 subunit (δ434-969)
      OverviewN-terminal GST-tagged, recombinant human NFkB, p50 subunit (D434-969), expressed in E. coli. Purified using glutathione agarose chromatography. MW = 74kDa.
      References
      Product Information
      Presentation50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.1% βmercaptoethanol, 0.03% Brij-35, 0.2mM PMSF, 1mM benzamidine and 50% glycerol
      Quality LevelMQ100
      Applications
      ApplicationN-terminal GST-tagged, recombinant human NFkB, p50 subunit (D434-969), expressed in E. coli. Purified using glutathione agarose chromatography.
      Application NotesDNA Binding Assay
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth.
      Gene Symbol
      • NFKB1
      • NF-kappa-B
      • DKFZp686C01211
      • MGC54151
      • NFKB-p105
      • NFKB-p50
      • EBP-1
      • KBF1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P19838 # Appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. Plays a role in the regulation of apoptosis.
      SIZE: 968 amino acids; 105356 Da
      SUBUNIT: Active NF-kappa-B is a heterodimer of an about 50 kDa DNA-binding subunit and the weak DNA-binding subunit p65. Two heterodimers might form a labile tetramer. Also interacts with MAP3K8. NF-kappa-B p50 subunit interacts with NCOA3 coactivator, which may coactivate NF-kappa-B dependent expression via its histone acetyltransferase activity. Interacts with DSIPI; this interaction prevents nuclear translocation and DNA-binding. Interacts with SPAG9 and UNC5CL.
      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
      DOMAIN: SwissProt: P19838 The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation. & Glycine-rich region (GRR) appears to be a critical element in the generation of p50.
      PTM: While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p50 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing. & Polyubiquitination seems to allow p105 processing.
      SIMILARITY: Contains 7 ANK repeats. & Contains 1 death domain. & Contains 1 RHD (Rel-like) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated in a DNA binding assay
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      13-118 04053252371875

      Documentation

      GST-NFκB p50 subunit (δ434-969) Certificates of Analysis

      TitleLot Number
      GST-NFkB p50 subunit (D434-969) - DAM1520788 DAM1520788
      GST-NFkappa;B p50 subunit (Delta;434-969) - 26844 26844
      GST-NFκB p50 subunit (Δ434-969) (recombinant protein expressed in E. coli) - 2384037 2384037
      GST-NFκB p50 subunit (Δ434-969) (recombinant protein expressed in E. coli) 3054689
      GST-NFκB p50 subunit (Δ434-969) (recombinant protein expressed in E. coli) 2879688
      GST-NFκB p50 subunit (Δ434-969) - 3541438 3541438
      GST-NFκB p50 subunit (Δ434-969) - 3991569 3991569
      GST-NFκB p50 subunit (Δ434-969) - 4163778 4163778