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CC327-2 PhotoHA®-LAP, Methacrylated Hyaluronic Acid Hydrogel Kit

Photocrosslinked hyaluronic acid hydrogel used for 3D cell culture and as a bioink for bioprinting (CC327 Kit Component 2)

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CC327-2
1 Kit  
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      Overview

      Replacement Information
      Description
      Catalogue NumberCC327-2
      DescriptionPhotoHA®-LAP, Methacrylated Hyaluronic Acid Hydrogel Kit
      Overview3D cell culture, including bioprinting, allows for the creation of more physiological cell models by allowing cells to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways not activated in traditional 2D cell culture methods. Hydrogels are water swollen polymers that allow for the culture of cells in 3-dimensions and can have profound effects on cellular development, differentiation, migration, and function. New areas of tissue engineering such as 3D bioprinting, have utilized UV photocrosslinked methacrylated hydrogel biomaterials (PEGMA, GelMA, HAMA and ColMA etc.) to encapsulate cells to make printable bioinks.

      Hyaluronic acid is the most abundant glycosaminoglycan in the body being an important component of several tissues throughout the body. While it is abundant in extracellular matrices, hyaluronan also contributes to tissue hydrodynamics, movement and proliferation of cells, and participates in a number of cell surface receptor interactions. Hyaluronic acid is a polymer of disaccharides, themselves composed of D-glucuronic acid and Nacetyl- D-glucosamine, linked via alternating β-(1→4) and β-(1→3) glycosidic bonds.

      The PhotoHA®-LAP, Methacrylated Hyaluronic Acid Hydrogel Kit (CC327: CC327-1, CC327-2) is based upon purified hyaluronic acid methacrylate (HAMA), which when photocrosslinked provides a native-like 3D environment for cells. In addition to hyaluronic acid methacrylate, the kit includes the photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) for users to easily fine tune their photocrosslinking experiments (i.e. altering hydrogel stiffness or gelling speeds).

      PhotoHA® is a registered trademark of Advanced BioMatrix, Inc.
      Alternate Names
      • HAMA
      • Hyaluronan
      • Hyaluronic Acid Hydrogel
      • Hyaluronic Acid Bioink
      • Hyaluronic Acid Methacrylate
      References
      Product Information
      Components
      • CC327-2 (Store at -20°C): Methacrylated Hyaluronic Acid, 1 X 100 mg (CS226446).
      • Note: The complete PhotoHA®-LAP, Methacrylated Hyaluronic Acid Hydrogel Kit (CC327) consists of CC327-1 and CC327-2 sold seperately.
      Applications
      ApplicationPhotocrosslinked hyaluronic acid hydrogel used for 3D cell culture and as a bioink for bioprinting (CC327 Kit Component 2)
      Key Applications
      • Cell Culture
      Application NotesPhotocrosslinking is initiated using a 405 nm blue light source.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceAppearance: Lyophilized Powder
      Sterility (USP modified): No Growth
      Grafting Efficiency: 50-70%
      pH: 6.0-8.0
      Osmolality: 200-400 mOsmo H20/kg in 1X PBS
      Molecular Weight: 100 – 150 kDa
      NMR: Characteristic
      Cell Compatibility: Characteristic
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 Kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CC327-2 04061842348183