Millipore Sigma Vibrant Logo

540221 Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: PNP

View Products on Sigmaaldrich.com
540221
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
540221-1MG
Retrieving availability...
—
Fulfillment and delivery delayed Fulfillment and delivery delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Plastic ampoule 1 mg
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Description
      OverviewRecombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorolysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides. This reaction is reversible. The human enzyme is a target for therapeutic immune response intervention.
      Catalogue Number540221
      Brand Family Calbiochem®
      SynonymsPNP
      References
      ReferencesFilgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun. 309, 917.
      Silva, R.G., et al. 2003. Protein Expr. Purif. 27, 158.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of P<sub>i</sub> into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.
      FormLiquid
      FormulationIn 50 mM Tris-HCl, pH 7.6.
      Quality LevelMQ100
      Applications
      Biological Information
      PuritySingle band by SDS-PAGE
      Specific Activity≥25 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      540221-1MG 04055977268751

      Documentation

      Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      540221

      References

      Reference overview
      Filgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun. 309, 917.
      Silva, R.G., et al. 2003. Protein Expr. Purif. 27, 158.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-August-2024 JSW
      SynonymsPNP
      DescriptionRecombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorlysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides in a reversible reaction. Human PNP is a target for therapeutic T-cell immune response intervention. Useful in the detection of inorganic phosphate from biochemical reactions.
      FormLiquid
      FormulationIn 50 mM Tris-HCl, pH 7.6.
      PuritySingle band by SDS-PAGE
      Specific activity≥25 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of Pi into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling
      ReferencesFilgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun. 309, 917.
      Silva, R.G., et al. 2003. Protein Expr. Purif. 27, 158.