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14-745 TAO3 Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, human TAO3 amino acids 1-411, N47S. For use in Kinase Assays.

TAO3 Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-745
10 µg  
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      Overview

      Replacement Information
      Description
      Catalogue Number14-745
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionTAO3 Protein, active, 10 µg
      OverviewN-terminal 6His-tagged, recombinant, human TAO3 amino acids 1-411; N47S.
      References
      Product Information
      Presentation10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal 6His-tagged, recombinant, human TAO3 amino acids 1-411, N47S. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      PurityPurity 84% by SDS-PAGE and Coomassie blue staining.
      SourceRecombinant, human TAO3 amino acids 1-411; N47S. Expressed by baculovirus in Sf21 cells.
      Specific Activity915U/mg, where one unit of TAO3 activity is defined as 1nmol phosphate incorporated into 0.8mg/ml myelin basic protein per minute at 30°C with a final ATP concentration of 100μM.
      Entrez Gene Number
      Gene Symbol
      • TAOK3
      • JIK
      • hKFC-A
      • MAP3K18
      • DPK
      • KDS
      • DKFZp666H245
      • FLJ31808
      Protein TargetTAO3
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilySTE
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9H2K8 # Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK.
      SIZE: 898 amino acids; 105406 Da
      SUBUNIT: Self-associates.
      SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Peripheral membrane protein. Note=Also localized to the peripheral cell membrane.
      TISSUE SPECIFICITY: Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver.
      PTM: Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: Q9H2K8 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 protein kinase domain.
      Molecular Weight50.2 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein.
      Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of TAO3.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
      Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-745 04053252408267

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      Alternative Packsize

      Catalogue Number Description  
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      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases