Anti-Aquaporin 3 (275-292), rabbit polyclonal, recognizes the ~40 and ~29 kDa forms of aquaporin-3 in rat kidney membrane. It is validated for Western blotting and immunohistochemistry.
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Anti-Aquaporin 3 (275-292) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Recognizes the ~40 kDa and ~29 kDa forms of aquaporin-3 in rat kidney membrane. Supplied with a control peptide (immunogen).
Catalogue Number
178613
Brand Family
Calbiochem®
Synonyms
Anti-AQP3, Anti-GLIP
Application Data
Detection of rat aquaporin 3 by immunoblotting. Sample: Extract from rat kidney membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (1:200). Detection: chemiluminescence. Detection of rat aquaporin 3 by immunohistochemistry. Samples: Longitudinal section of rat colon showing mucosa (M), submucosa (SM), and muscularis externa (ME). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (Dilution Assay Dependent). Detection: fluorescence.
References
References
Echevarria, M., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 10997. Ishibashi, K., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 6269.
Product Information
Form
Lyophilized
Formulation
50 µl antibody lyophilized from PBS, 1% BSA, 5% sucrose, pH. 7.4 and 40 µg AQP3275-292 control peptide lyophilized from PBS.
Immunoblotting (1:200) Immunohistochemistry (assay dependent; see application references)
Application Comments
For use as a negative control, preincubate 1 µg of peptide with 1 µg of antibody for 1 h at room temperature. The epitope is unique for AQP3 and is not present in other known related proteins. It is highly homologous in mouse (17/18 identical) and human (15/18 identical) AQP3 protein. Recognition of AQP3 can be fully inhibited with the soluble AQP3275-292 blocking peptide. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogen
a synthetic peptide [(KY)STEAENVKLAH(Nle)KHKEQI] corresponding to amino acids 275-292 of rat AQP3, with norleucine replacing methionine, conjugated to KLH
Immunogen
Rat
Host
Rabbit
Isotype
IgG
Species Reactivity
Rat
Antibody Type
Polyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute the antibody with 50 µl deionized water to make a 300 µg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Reconstitue the peptide with 100 µl deionized water, aliquot, and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Documentation
Anti-Aquaporin 3 (275-292) Rabbit pAb Certificates of Analysis
Title
Lot Number
178613
References
Reference overview
Echevarria, M., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 10997. Ishibashi, K., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 6269.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
02-November-2009 RFH
Synonyms
Anti-AQP3, Anti-GLIP
Application
Immunoblotting (1:200) Immunohistochemistry (assay dependent; see application references)
Application Data
Detection of rat aquaporin 3 by immunoblotting. Sample: Extract from rat kidney membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (1:200). Detection: chemiluminescence. Detection of rat aquaporin 3 by immunohistochemistry. Samples: Longitudinal section of rat colon showing mucosa (M), submucosa (SM), and muscularis externa (ME). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (Dilution Assay Dependent). Detection: fluorescence.
Description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40 kDa and ~29 kDa aquaporin-3 proteins. Supplied with a control peptide (immunogen).
Background
AQP3 may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It is also slightly permeable to urea and glycerol. AQP3 may play an important role in gastrointestinal tract water transport and in glycerol metabolism. AQP3 has a wide tissue distribution. In the kidney it is predominantly expressed in the inner medulla.
Host
Rabbit
Immunogen species
Rat
Immunogen
a synthetic peptide [(KY)STEAENVKLAH(Nle)KHKEQI] corresponding to amino acids 275-292 of rat AQP3, with norleucine replacing methionine, conjugated to KLH
Isotype
IgG
Species
rat
Form
Lyophilized
Formulation
50 µl antibody lyophilized from PBS, 1% BSA, 5% sucrose, pH. 7.4 and 40 µg AQP3275-292 control peptide lyophilized from PBS.
Preservative
≤0.1% sodium azide
Comments
For use as a negative control, preincubate 1 µg of peptide with 1 µg of antibody for 1 h at room temperature. The epitope is unique for AQP3 and is not present in other known related proteins. It is highly homologous in mouse (17/18 identical) and human (15/18 identical) AQP3 protein. Recognition of AQP3 can be fully inhibited with the soluble AQP3275-292 blocking peptide. Variables associated with assay conditions will dictate the optimal working dilution.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute the antibody with 50 µl deionized water to make a 300 µg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Reconstitue the peptide with 100 µl deionized water, aliquot, and freeze (-20°C).
Toxicity
Standard Handling
References
Echevarria, M., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 10997. Ishibashi, K., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 6269.
Application references
Immunohistochemistry
Ishibashi, K., et al. 1997. Am. J. Physiol. 272, F235.
Ecelbarger, C.A., et al. 1995. Am. J. Physiol. 269, F663.
