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217660 Anti-CD18 Mouse mAb (IB4)

217660
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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217660-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.
      Catalogue Number217660
      Brand Family Calbiochem®
      SynonymsAnti-β2 Integrin
      References
      ReferencesBerman, M.E., et al. 1996. J. Immunol. 156, 1515.
      Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
      Mazzone, A., and Ricevuti, G. 1995. Haematologica 80, 161.
      Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
      Landis, R.C., et al. 1994. J. Cell Biol. 126, 529.
      Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 5699.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Negative controlNalm-6 cells
      Positive controlHPB-MLT cells
      Preservative0.5 mg/ml gentamicin sulfate
      Quality LevelMQ100
      Applications
      Key Applications Immunoprecipitation
      Cell Inhibition Assays
      Flow Cytometry
      Frozen Sections
      Application NotesFlow Cytometry (5-10 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      Cell Inhibition Assays (see comments)
      Application CommentsBlocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenhuman peripheral blood monocytes
      ImmunogenHuman
      CloneIB4
      HostMouse
      Purity≥95% by SDS-PAGE
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-CD18 Mouse mAb (IB4) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD18 Mouse mAb (IB4) Certificates of Analysis

      TitleLot Number
      217660

      References

      Reference overview
      Berman, M.E., et al. 1996. J. Immunol. 156, 1515.
      Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
      Mazzone, A., and Ricevuti, G. 1995. Haematologica 80, 161.
      Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
      Landis, R.C., et al. 1994. J. Cell Biol. 126, 529.
      Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 5699.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-July-2009 JSW
      SynonymsAnti-β2 Integrin
      ApplicationFlow Cytometry (5-10 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      Cell Inhibition Assays (see comments)
      DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18).
      BackgroundHuman CD18 (β2 integrin) is expressed on leukocytes as part of a heterodimeric complex with either CD11a, b or c (integrins αL, αM, or αX). CD18/CD11 heterodimers function in cell adhesion in immune and inflammatory responses.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman peripheral blood monocytes
      CloneIB4
      IsotypeIgG2a
      Specieshuman
      Positive controlHPB-MLT cells
      Negative controlNalm-6 cells
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥95% by SDS-PAGE
      Preservative0.5 mg/ml gentamicin sulfate
      CommentsBlocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesBerman, M.E., et al. 1996. J. Immunol. 156, 1515.
      Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
      Mazzone, A., and Ricevuti, G. 1995. Haematologica 80, 161.
      Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
      Landis, R.C., et al. 1994. J. Cell Biol. 126, 529.
      Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 5699.