374089 Anti-Heme Oxygenase-1 (1-261) Rabbit pAb

374089
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~32 kDa HO-1. Does not cross-react with HO-2.
      Catalogue Number374089
      Brand Family Calbiochem®
      SynonymsAnti-HO-1, Anti-Hsp32
      Application Data
      Detection of heme oxygenase-1 by immunoblotting. Samples: Purified SPP-730 (lane 1), extracts from human liver microsomes (lane 2), rat liver microsomes (lane 3), mouse liver microsomes (lane 4), and canine liver microsomes (lane 5). Primary antibody: Anti-Heme Oxygenase-1 (1-261) Rabbit pAb (Cat. No. 374089) (1:1000). Detection: chemiluminescence.

      Detection of human heme oxygenase-1 by immunohistochemistry. Sample: Alzheimer's disease brain tissue. Primary antibody: Anti-Heme Oxygenase-1 (1-261) Rabbit pAb (Cat. No. 374089) (assay dependent). Detection:
      References
      ReferencesSmith, M., et al. 1994. Am. J. Path. 145, 42.
      Shibahara, S., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 7865.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Positive controlRecombinant rat HO-1 (Hsp32) protein or recombinant human HO-1 (Hsp32) protein
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunohistochemistry
      Immunoprecipitation
      Flow Cytometry
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesFlow Cytometry (see comments)
      Immunoblotting (1:500, colorimetric; 1:2000, chemiluminescence)
      Immunohistochemistry (see comments)
      Immunoprecipitation (1:100)
      Immunocytochemistry (see comments)
      Application CommentsMay cross-react with HO-2 in immunoblotting. The signal is weaker in total cell lysates due to the low abundance of HO-1. Additionally, the antibody recognizes a ~50 kDa band present only in transformed cell lines, not in normal tissues. This antibody has also been reported to work in immunohistochemistry, immunoelectron microscopy, and flow cytometry. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenrecombinant rat HO-1 consisting amino acids 1-261 (lacks the membrane spacing region)
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C or -70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Heme Oxygenase-1 (1-261) Rabbit pAb Certificates of Analysis

      TitleLot Number
      374089

      References

      Reference overview
      Smith, M., et al. 1994. Am. J. Path. 145, 42.
      Shibahara, S., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 7865.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-March-2008 JSW
      SynonymsAnti-HO-1, Anti-Hsp32
      ApplicationFlow Cytometry (see comments)
      Immunoblotting (1:500, colorimetric; 1:2000, chemiluminescence)
      Immunohistochemistry (see comments)
      Immunoprecipitation (1:100)
      Immunocytochemistry (see comments)
      Application Data
      Detection of heme oxygenase-1 by immunoblotting. Samples: Purified SPP-730 (lane 1), extracts from human liver microsomes (lane 2), rat liver microsomes (lane 3), mouse liver microsomes (lane 4), and canine liver microsomes (lane 5). Primary antibody: Anti-Heme Oxygenase-1 (1-261) Rabbit pAb (Cat. No. 374089) (1:1000). Detection: chemiluminescence.

      Detection of human heme oxygenase-1 by immunohistochemistry. Sample: Alzheimer's disease brain tissue. Primary antibody: Anti-Heme Oxygenase-1 (1-261) Rabbit pAb (Cat. No. 374089) (assay dependent). Detection:
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~32 kDa HO-1 protein.
      BackgroundHeme oxygenase (HO) is a microsomal enzyme that oxidatively cleaves heme, a pro-oxidant, into carbon monoxide and biliverdin, which is reduced to the antioxidant, bilirubin. Two isozymes, termed HO-1 and HO-2, have been identified. By SDS-PAGE, mammalian HO-1 is ~31-33 kDa; HO-2 is ~36 kDa. HO-1, also referred to as stress protein Hsp32, is the inducible form of the enzyme. HO-1 expression can be induced by a variety of stimuli such as heme, metals, hormones, UV radiation and sulfhydryl depleting agents. In contrast, HO-2 is constitutively expressed. HO-2 is the most prevalent form in most tissues, except the spleen where HO-1 levels are predominant.
      HostRabbit
      Immunogen speciesRat
      Immunogenrecombinant rat HO-1 consisting amino acids 1-261 (lacks the membrane spacing region)
      IsotypeIgG
      Speciescanine, guinea pig, hamster, human, monkey, mouse, rabbit, rat, sheep
      Positive controlRecombinant rat HO-1 (Hsp32) protein or recombinant human HO-1 (Hsp32) protein
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsMay cross-react with HO-2 in immunoblotting. The signal is weaker in total cell lysates due to the low abundance of HO-1. Additionally, the antibody recognizes a ~50 kDa band present only in transformed cell lines, not in normal tissues. This antibody has also been reported to work in immunohistochemistry, immunoelectron microscopy, and flow cytometry. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C or -70°C).
      Toxicity Standard Handling
      ReferencesSmith, M., et al. 1994. Am. J. Path. 145, 42.
      Shibahara, S., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 7865.