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05-1000 Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1)

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05-1000
100 µg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
VrtELISA, FC, IF, IH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1000
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Phosphoserine Antibody, clone 4A4 (mouse IgG1)
Background InformationThe identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (>95%).
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified mouse monoclonal IgG1 in buffer containing PBS with 0.1% sodium azide and 30% glycerol.
Quality LevelMQ100
Applications
ApplicationDetect Phosphoserine using this Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) validated for use in ELISA, FC, IF, IH(P) & WB.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunofluorescence

Flow Cytometry

Immunohistochemistry (Paraffin)

ELISA
Biological Information
ImmunogenPhosphoserine coupled to KLH.
Clone4A4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes serine-phosphorylated proteins from all species.
IsotypeIgG1
Species Reactivity
  • Vertebrates
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein G-Sepharose™ Chromatography
Molecular WeightDependent upon the molecular weight of the serine phosphorylated protein being detected.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.

Western Blot Analysis:
0.5–2 μg/mL of this lot detected serinephosphorylated proteins in a lysate from either insulin or Calyculin A/Okadaic-treated human A431 carcinoma cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
For maximum recovery, centrifuge the original vial prior to cap removal. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-1000 04053252586132

Documentation

Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) SDS

Title

Safety Data Sheet (SDS) 

Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) Certificates of Analysis

TitleLot Number
Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 2137995 2137995
Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 2433477 2433477
Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 2023922 2023922
Anti-Phosphoserine, clone 4A4 (Mouse monoclonal IgG1) - 4080114 4080114
Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 1964542 1964542
Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 2016023 2016023
Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 2201580 2201580
Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 2326373 2326373
Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 2890779 2890779
Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 3280139 3280139

References

Reference overviewApplicationPub Med ID
A phosphorylation switch controls the spatiotemporal activation of Rho GTPases in directional cell migration.
Cao, X; Kaneko, T; Li, JS; Liu, AD; Voss, C; Li, SS
Nature communications  6  7721  2015

Show Abstract
26166433 26166433
Nucleocytoplasmic shuttling of a GATA transcription factor functions as a development timer.
Cai, H; Katoh-Kurasawa, M; Muramoto, T; Santhanam, B; Long, Y; Li, L; Ueda, M; Iglesias, PA; Shaulsky, G; Devreotes, PN
Science (New York, N.Y.)  343  1249531  2014

Show Abstract
Western Blotting24653039 24653039
Epidermal growth factor promotes protein degradation of epithelial protein lost in neoplasm (EPLIN), a putative metastasis suppressor, during epithelial-mesenchymal transition.
Zhang, S; Wang, X; Iqbal, S; Wang, Y; Osunkoya, AO; Chen, Z; Chen, Z; Shin, DM; Yuan, H; Wang, YA; Zhau, HE; Chung, LW; Ritenour, C; Kucuk, O; Wu, D
The Journal of biological chemistry  288  1469-79  2013

Show Abstract
23188829 23188829
BAF complexes facilitate decatenation of DNA by topoisomerase IIα.
Dykhuizen, EC; Hargreaves, DC; Miller, EL; Cui, K; Korshunov, A; Kool, M; Pfister, S; Cho, YJ; Zhao, K; Crabtree, GR
Nature  497  624-7  2013

Show Abstract
23698369 23698369
Estrous cycle variations in GABA(A) receptor phosphorylation enable rapid modulation by anabolic androgenic steroids in the medial preoptic area.
Oberlander, JG; Porter, DM; Onakomaiya, MM; Penatti, CA; Vithlani, M; Moss, SJ; Clark, AS; Henderson, LP
Neuroscience  226  397-410  2012

Show Abstract
22989919 22989919
Increased NADPH oxidase-derived superoxide is involved in the neuronal cell death induced by hypoxia-ischemia in neonatal hippocampal slice cultures.
Lu, Q; Wainwright, MS; Harris, VA; Aggarwal, S; Hou, Y; Rau, T; Poulsen, DJ; Black, SM
Free radical biology & medicine  53  1139-51  2012

Show Abstract
22728269 22728269
5-Aza-2'-deoxycytidine reactivates gene expression via degradation of pRb pocket proteins.
Zheng, Z; Li, L; Liu, X; Wang, D; Tu, B; Wang, L; Wang, H; Zhu, WG
FASEB journal : official publication of the Federation of American Societies for Experimental Biology  26  449-59  2012

Show Abstract
21990374 21990374
Osmotic stress stimulates phosphorylation and cellular expression of heat shock proteins in rhesus macaque sperm.
Cole JA, Meyers SA
Journal of andrology  32  402-10. Epub 2010 Nov 18.  2011

Show Abstract
21088232 21088232
Glycine receptor internalization by protein kinases activation.
Miguel Ángel Velázquez-Flores,Rocío Salceda,Miguel Ángel Velázquez-Flores,Rocío Salceda
Synapse (New York, N.Y.)  65  2011

Show Abstract
21656573 21656573
CDK11p58 phosphorylation of PAK1 Ser174 promotes DLC2 binding and roles on cell cycle progression.
Kong, Xiangfei, et al.
J. Biochem., 146: 417-27 (2009)  2009

Show Abstract
19520772 19520772

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Catalogue Number Description
05-1000X Anti-Phosphoserine Antibody, clone 4A4

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies