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235706 CSK, GST-Fusion, Human, Recombinant, S. frugiperda

235706
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Full-length, recombinant, human CSK (Genbank accession number = NM_004383) fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. CSK is a cytoplasmic tyrosine kinase that regulates several cellular processes including downregulation of the tyrosine kinase activity of c-Src.
      Catalogue Number235706
      Brand Family Calbiochem®
      Synonymsc-Src Tyrosine Kinase
      Application Data
      Increasing concentrations of CSK were assayed as outlined above.
      References
      ReferencesCloutier, J. F. and Veillette, A. 1996. EMBO J. 15, 4909.
      Armstrong, E., et al. 1992. Cytogenet. Cell Genet. 60, 119.
      Partanen, J., et al. 1991. Oncogene 6, 2013.
      Product Information
      Unit of DefinitionKinase activity is measured as the nanomolar amount of phosphate incorporated into Poly(Glu-Tyr) substrate at 30°C using a concentration of 50 µM ATP (0.83 µCi/assay).
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM EGTA, 0.1 mM PMSF, 25% glycerol, pH 7.5.
      Applications
      Biological Information
      Purity>85% by SDS-PAGE
      Specific Activity≥362 nmol/min/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      CSK, GST-Fusion, Human, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      235706

      References

      Reference overview
      Cloutier, J. F. and Veillette, A. 1996. EMBO J. 15, 4909.
      Armstrong, E., et al. 1992. Cytogenet. Cell Genet. 60, 119.
      Partanen, J., et al. 1991. Oncogene 6, 2013.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 JSW
      Synonymsc-Src Tyrosine Kinase
      Application Data
      Increasing concentrations of CSK were assayed as outlined above.
      DescriptionFull-length, recombinant, human CSK (Genbank accession number = NM_004383) fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. CSK is a cytoplasmic tyrosine kinase that regulates several cellular processes including downregulation of the tyrosine kinase activity of c-Src.
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM EGTA, 0.1 mM PMSF, 25% glycerol, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Kinase assay conditions: Dilute purified CSK in assay dilution buffer (5 mM MnCl2, 4 mM MOPS, 4 mM MgCl2, 2.5 mM β-glycerophosphate, 50 µM DTT, pH 7.2) to the desired concentration. Mix 10 µl diluted enzyme, 5 µl Poly(Glu-Tyr) substrate (1 mg/ml stock), and 5 µl H2O and incubate for 5 min at 30°C. Add 5 µl [32P] ATP mixture (250 µM ATP, 0.16 µCi/µl in 4X assay dilution buffer) and incubate for 15 min at 30°C. Terminate the reaction by spotting 20 µl of the reaction mixture onto a phosphocellulose P81 paper, dry, and wash several times with 1% phosphoric acid prior to counting in the presence of scintillation fluid.
      Purity>85% by SDS-PAGE
      Specific activity≥362 nmol/min/mg protein
      Unit definitionKinase activity is measured as the nanomolar amount of phosphate incorporated into Poly(Glu-Tyr) substrate at 30°C using a concentration of 50 µM ATP (0.83 µCi/assay).
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesCloutier, J. F. and Veillette, A. 1996. EMBO J. 15, 4909.
      Armstrong, E., et al. 1992. Cytogenet. Cell Genet. 60, 119.
      Partanen, J., et al. 1991. Oncogene 6, 2013.