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208748 Calpain-1 Substrate, Fluorogenic - Calbiochem

208748
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208748-2MG
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      Description
      OverviewAn internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (Km = 4.6 µM; kcat = 11 s-1). Cleavage occurs between Tyr-Gly residues and results in enhanced fluorescence.
      Catalogue Number208748
      Brand Family Calbiochem®
      Synonymsμ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH
      References
      ReferencesMittoo, S., et al. 2003. Anal. Biochem. 319, 234.
      Product Information
      ATP CompetitiveN
      FormRed lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₇₉H₉₅N₁₃O₁₉S₂
      Chemical formulaC₇₉H₉₅N₁₃O₁₉S₂
      Hygroscopic Hygroscopic
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetA sensitive and specific substrate for calpain-1
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Peptide SequenceH-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Calpain-1 Substrate, Fluorogenic - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Calpain-1 Substrate, Fluorogenic - Calbiochem Certificates of Analysis

      TitleLot Number
      208748

      References

      Reference overview
      Mittoo, S., et al. 2003. Anal. Biochem. 319, 234.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-May-2008 RFH
      Synonymsμ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH
      DescriptionAn internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (Km = 4.6 µM; kcat = 11 s-1). Cleavage occurs at the Tyr-Gly bond and results in enhanced fluorescence. Excitation max: ~490 nm; emission max: ~518 nm.
      FormRed lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Recommended reaction conditions50 mM Tris-HCl, 50 mM NaCl, 5 mM β-mercaptoethanol, 5 mM CaCl2, 1 mM EDTA, 1 mM EGTA, and 1 µM substrate, pH 7.5.
      Chemical formulaC₇₉H₉₅N₁₃O₁₉S₂
      Peptide SequenceH-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH
      Purity≥95% by HPLC
      SolubilityDMSO (1 mg/ml)
      Storage -20°C
      Hygroscopic
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesMittoo, S., et al. 2003. Anal. Biochem. 319, 234.