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218709 Casein Kinase II, α2, Active, Human, Recombinant, E. coli

218709
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Recombinant, human casein kinase II, α2, (CKII, α2) fused to maltose binding protein (MBP) at the N-terminus and expressed in E. coli. CKII, α2 is a catalytic subunit of the serine/threonine protein kinase CKII. It is suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.

      Catalogue Number218709
      Brand Family Calbiochem®
      SynonymsCK2α2, CSNK2A2
      Application Data
      Increasing concentrations of Casein Kinase II, α2 were assayed according to the protocol outlined above.
      References
      ReferencesAntonelli, M., et al. 1997. Eur. J. Biochem. 241, 272.
      Xu, X., et al. 1997. Genomics 48, 79.
      Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to RRRADDSDDDDD peptide substrate per min at 30°C.
      FormLiquid
      FormulationIn 50 mM Tris-HCl, 500 mM NaCl, 10 mM DTT, pH 8.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥ 90% by SDS PAGE
      Specific Activity≥841.819 Units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Casein Kinase II, α2, Active, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Casein Kinase II, α2, Active, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      218709

      References

      Reference overview
      Antonelli, M., et al. 1997. Eur. J. Biochem. 241, 272.
      Xu, X., et al. 1997. Genomics 48, 79.
      Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-April-2017 JSW
      SynonymsCK2α2, CSNK2A2
      Application Data
      Increasing concentrations of Casein Kinase II, α2 were assayed according to the protocol outlined above.
      DescriptionRecombinant, human casein kinase II, α2, (CKII, α2) fused to maltose binding protein (MBP) at the N-terminus and expressed in E. coli. CKII, α2 is a catalytic subunit of the serine/threonine protein kinase CKII. It is suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.
      FormLiquid
      FormulationIn 50 mM Tris-HCl, 500 mM NaCl, 10 mM DTT, pH 8.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Kinase Activity Assay Required Materials •Assay Buffer: 25 mM β-glycerophosphate, 20 mM Tris-HCl, 5 mM EGTA, 1 mM DTT, pH 7.5 •Substrate: 800 µM RRRDDDSDDD peptide substrate •CKII, α2: 5-10 ng/µl CKII, α2 diluted in Assay Buffer just prior to use •Magnesium/ATP Mix: 75 mM MgCl2, 500 µM ATP •Diluted [γ-32P]ATP/Magnesium/ATP Mix: Mix 197 µl Magnesium/ATP Mix with 3 µl (30 µCi) [γ-32P]ATP (3000 Ci/mmol) Protocol 1. Pipette 10 µl Assay Buffer into a clean microcentrifuge tube on ice. 2. Add 10 µl Substrate. 3. Add 10 µl diluted CKII, α2. 4. Add 10 µl Diluted [γ-32P]ATP/Magnesium/ATP Mix. 5. Incubate at 37° for 10 min. 6. Stop the reaction by placing the tubes on ice. 7. Spot 10 µl of each reaction on P81 paper and allow to bind for 30 s. 8. Wash by immersing the paper in 0.75% phosphoric acid and gently shaking. Repeat for a total of 3 washes. 9. Wash as in step 8 using acetone. 10. Dry using an infrared light. 11. Determine the cpm using a scintillation counter or imager.
      Purity≥ 90% by SDS PAGE
      Specific activity≥841.819 Units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to RRRADDSDDDDD peptide substrate per min at 30°C.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesAntonelli, M., et al. 1997. Eur. J. Biochem. 241, 272.
      Xu, X., et al. 1997. Genomics 48, 79.
      Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.