218704 Casein Kinase II, α-Subunit, Human, Recombinant, E. coli

218704
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Recombinant, human casein kinase II α-subunit expressed in E. coli. The catalytic subunit of serine/threonine protein kinase CKII. Suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.

      Note: 1 mU = 1 milliunit.
      Catalogue Number218704
      Brand Family Calbiochem®
      SynonymsrhCKIIα, Protein Kinase CKIIα
      References
      ReferencesAllende, J.E., and Allende, C.C. 1995. FASEB J. 9, 313.
      Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
      Issinger, O.-G. 1993. Pharmacol. Ther. 59, 1.
      Grankowski, N., et al. 1991. Eur. J. Biochem. 198, 25.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 µmol phosphate from ATP to the synthetic casein kinase II substrate RRRDDDSDDD per min at 37°C, pH 8.5.
      FormLiquid
      FormulationIn 500 mM NaCl, 25 mM Tris-HCl, 1 mM DTT, 500 µM PMSF, 50% glycerol, pH 8.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Specific Activity≥1.0 unit/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Contaminantsslight autoproteolysis yields one additional band of 39 kDa; however, no loss in activity is associated with the appearance of this 39 kDa product
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Casein Kinase II, α-Subunit, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Casein Kinase II, α-Subunit, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      218704

      References

      Reference overview
      Allende, J.E., and Allende, C.C. 1995. FASEB J. 9, 313.
      Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
      Issinger, O.-G. 1993. Pharmacol. Ther. 59, 1.
      Grankowski, N., et al. 1991. Eur. J. Biochem. 198, 25.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-May-2008 JSW
      SynonymsrhCKIIα, Protein Kinase CKIIα
      DescriptionRecombinant, human casein kinase II α-subunit expressed in E. coli. The catalytic subunit of serine/threonine protein kinase CKII. Suitable for use in reactions where the autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt sensitive with respect to its activity. Easily inactivated by treatment with heat, trypsin digestion, or urea.
      FormLiquid
      FormulationIn 500 mM NaCl, 25 mM Tris-HCl, 1 mM DTT, 500 µM PMSF, 50% glycerol, pH 8.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥95% by SDS-PAGE
      Contaminantsslight autoproteolysis yields one additional band of 39 kDa; however, no loss in activity is associated with the appearance of this 39 kDa product
      Specific activity≥1.0 unit/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 µmol phosphate from ATP to the synthetic casein kinase II substrate RRRDDDSDDD per min at 37°C, pH 8.5.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesAllende, J.E., and Allende, C.C. 1995. FASEB J. 9, 313.
      Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
      Issinger, O.-G. 1993. Pharmacol. Ther. 59, 1.
      Grankowski, N., et al. 1991. Eur. J. Biochem. 198, 25.