219344 Cathepsin S, Human Spleen

219344
  
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      Overview

      Replacement Information

      Key Spec Table

      Description
      OverviewNative cathepsin S from human spleen. A major lysosomal cysteine proteinase that mediates degradation of MHC class II-invariant chain complexes in human and mouse antigen presenting cells. Cathepsin S secreted by activated macrophages has the ability to process myelin basic protein and amyloid β peptide which may be relevant to the pathogenesis of Alzheimer′s disease. It can hydrolyze elastin at both acidic and neutral pH.

      This product has been discontinued.





      Note: 1 mU = 1 milliunit.
      Catalogue Number219344
      Brand Family Calbiochem®
      References
      ReferencesLiuzzo, J.P., et al. 1999. Mol. Med. 5, 334.
      Riese, R.J., et al. 1998. J. Clin. Invest. 101, 2351.
      Sukhova, G.K., et al. 1998. J. Clin. Invest. 102, 576.
      Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol. 244, 500.
      Xin, X.Q., et al. 1992. Arch. Biochem. Biophys. 299, 334.
      Kirschke, H., et al. 1989. Biochem. J. 264, 467.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze Z-VVR-AMC in 10 min at 25°C, in 100 mM KPO₄, 5 mM EDTA, pH 6.5 (the assay is standardized such that 0.041 nmol AMC gives a reading of 1000 RFU.
      EC number3.4.22.27
      FormLyophilized
      FormulationLyophilized from 100 mM sodium acetate, 1 mM EDTA, pH 5.5.
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      SourcePrepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HBC, HCV, and HIV.
      Specific Activity≥50 mU/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Cathepsin S, Human Spleen SDS

      Title

      Safety Data Sheet (SDS) 

      Cathepsin S, Human Spleen Certificates of Analysis

      TitleLot Number
      219344

      References

      Reference overview
      Liuzzo, J.P., et al. 1999. Mol. Med. 5, 334.
      Riese, R.J., et al. 1998. J. Clin. Invest. 101, 2351.
      Sukhova, G.K., et al. 1998. J. Clin. Invest. 102, 576.
      Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol. 244, 500.
      Xin, X.Q., et al. 1992. Arch. Biochem. Biophys. 299, 334.
      Kirschke, H., et al. 1989. Biochem. J. 264, 467.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-September-2007 JSW
      DescriptionNative cathepsin S from human spleen. A major lysosomal cysteine protease preferentially expressed in macrophages and microglia. Mediates degradation of MHC Class II-invariant chain complexes in human and mouse antigen presenting cells. Cathepsin S secreted by activated macrophages may be relevant to the pathogenesis of Alzheimer's disease as a result of its ability to process myelin basic protein and β amyloid (Aβ) peptide. Cathepsin S can also hydrolyze elastin at both acidic and neutral pH.
      FormLyophilized
      FormulationLyophilized from 100 mM sodium acetate, 1 mM EDTA, pH 5.5.
      SourcePrepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HBC, HCV, and HIV.
      EC number3.4.22.27
      Purity≥90% by SDS-PAGE
      Specific activity≥50 mU/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze Z-VVR-AMC in 10 min at 25°C, in 100 mM KPO₄, 5 mM EDTA, pH 6.5 (the assay is standardized such that 0.041 nmol AMC gives a reading of 1000 RFU.
      SolubilityAqueous buffers. Please refer to the label for concentration prior to lyophilization.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
      Toxicity Standard Handling
      ReferencesLiuzzo, J.P., et al. 1999. Mol. Med. 5, 334.
      Riese, R.J., et al. 1998. J. Clin. Invest. 101, 2351.
      Sukhova, G.K., et al. 1998. J. Clin. Invest. 102, 576.
      Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol. 244, 500.
      Xin, X.Q., et al. 1992. Arch. Biochem. Biophys. 299, 334.
      Kirschke, H., et al. 1989. Biochem. J. 264, 467.