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238540 ChromoSave™ Reagent

238540
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.





An aqueous-based mounting medium designed for the permanent preservation of alkaline phosphatase and immunoperoxidase stained cells and tissue sections. ChromoSave™ Reagent can be used with organic solvent soluble horseradish peroxidase chromogens, such as 3-amino-9-ethylcarbazole (AEC) and 4-chloro-1-napthol, as well as alkaline phosphatase chromogens, such as 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium (BCIP/NBT) and Naphthol AS-MX phosphate/Fast Red TR salt. It can also be used with tissue sections stained with chromogens that are insoluble in organic solvents, such as diaminobenzidine (DAB), eliminating the dehydration step. ChromoSave™ Reagent is also compatible with fluorescein, rhodamine, and TEXAS RED® stained tissues. For preservation of phycoerythrin, phycocyanin, or allophycocyanin stained tissue samples, use FluorSave™ Reagent (Cat. No. 345789).
Catalogue Number238540
Brand Family Calbiochem®
References
Product Information
FormLiquid
FormulationContains 0.05% sodium azide.
Preservative≤0.1% sodium azide
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
238540 0

Documentation

ChromoSave™ Reagent Certificates of Analysis

TitleLot Number
238540
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-August-2008 JSW
DescriptionAn aqueous mounting medium designed for the permanent preservation of peroxidase-and alkaline phosphatase-stained tissue sections. Hardened ChromoSave™ forms an impervious barrier to organic solvent-based mounting media, such as Permount. When properly mounted with ChromoSave™, samples exhibit enhanced contrast and maintain their original clarity and strength of color. ChromoSave™ works well with organic solvent-soluble chromogens such as AEC, 4-chloro-1-naphthol, Naphthol AS-MX phosphate/Fast Red TR salt, and BCIP/NBT, as well as chromogens that are insoluble in organic solvents, such as DAB, thus eliminating the dehydration procedure. ChromoSave is also compatible with fluorescein-, rhodamine-, Texas Red-, and FluoroBlue-stained specimens. Not suitable for specimens stained with Phycoerythrin, Phycocyanin, or Allophycocyanin.
FormLiquid
FormulationContains 0.05% sodium azide.
Recommended reaction conditions
Do not use oil directly on top of dried ChromoSave. Instructions for mounting with ChromoSave™ Reagent 1. Before using this product, remove the red cap covering the tip of the bottle and cut off the tip of the nozzle using a sharp blade or scissors. Re-cap the bottle of ChromoSave™ and store the bottle upside down in a cup or bottle. This eliminates the introduction of air bubbles into the medium. 2. Place all slides to be mounted on a horizontal surface and apply three drops of ChromoSave™ to the tissue sections. 3. Rotate the slides covered by the ChromoSave™ so that it spreads to cover an area roughly the size of a quarter. Do not apply a coverslip on top of the ChromoSave™. 4. Place the slides horizontally in an oven at 70-80°C for at least 10 min or 20-37°C for 2-3 h. Note: when using fluorescent dye-stained specimens, bake at 60-70°C for 20 min. 5. Remove the slides from the oven and allow them to reach room temperature. The slides are now ready for microscopic visualization. Note: microscopic air bubbles may be found after applying ChromoSave™ to particular tissue sections, especially gastrointestinal sections or necrotic areas on the sections. Slow drying of the ChromoSave™ at room temperature may help eliminate this occurrence. Post-Mounting ChromoSave™ Reagent with Permount ChromoSave™ is not suitable for use directly with oil. Slides with hardened ChromoSave™ can be post-mounted and cover-slipped in Permount if desired. Place 3 drops of Permount on top of the hardened ChromoSave™ and apply a coverslip. Allow the slides to dry at room temperature. The hardened polymer in ChromoSave™ has a refractive index very close to that of Permount. Do not heat slides after the application of Permount. Removal of ChromoSave™ Reagent When required, solidified ChromoSave™ may be partially or completely removed only if the slides have not been post-mounted with Permount. Immerse the slides in distilled water and very gently agitate the slides to assist in the removal of the ChromoSave™ layer. Repeat this procedure until the film of ChromoSave™ is removed. If overnight incubations are required, add sodium azide to a concentration of 0.05% to avoid bacterial growth. Excessive soaking in water may result in the detachment of the tissue sections from the glass slides. Following removal of the ChromoSave™, treat the tissue sections with a buffer compatible with the staining procedure to be used. Failure to re-stain the slides should not be considered as negative staining since incomplete removal of the ChromoSave™ may interfere with some staining procedures. Variables associated with assay conditions will dictate the proper working dilution.
Preservative≤0.1% sodium azide
Storage +15°C to +30°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling