233157 Cleland’s REDUCTACRYL™ Reagent

233157
  
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      Overview

      Replacement Information

      Key Spec Table

      CAS #
      3483-12-3
      Description
      Overview

      This product has been discontinued.

      We are currently only offering non-immobilized DTT, Cleland's Reagent (Cat. No. 233155), Cleland's Reagent, ULTROL™ Grade (Cat. No. 233153), and Cleland's Reagent, Molecular Biology Grade (Cat. No. 233156). Please read the alternative product documentation carefully and contact technical service if you need additional information.





      Immobilized form of DTT on polyacrylamide resin, with applications similar to those of DTT itself (Cat. Nos. 233153, 233156, and 233155) but with the important advantage that no soluble components are added to the system. REDUCTACRYL™ Reagent can be used in a batch technique, in which the resin can be easily removed by filtration, or it can be packed into a column. Using a 10-fold excess of resin in the batch technique, it has been shown that 1 mM oxidized glutathione can be reduced completely to glutathione in 15 min at room temperature, pH 7.5.
      Reduction capacity: >0.06 meq/g of resin.
      Catalogue Number233157
      Brand Family Calbiochem®
      SynonymsCleland’s Reagent, Immobilized
      References
      Product Information
      CAS number3483-12-3
      FormOff-white solid
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSEK1610000
      Safety Information
      R PhraseR: 22-36/38

      Harmful if swallowed.
      Irritating to eyes and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Do not freeze Yes
      Special InstructionsUnstable in solution; reconstitute just prior to use. To regenerate, expose to sodium borohydride (100-200 mg/g resin) in H₂O for 15 min. Follow by washing with H₂O containing 1 M acetic acid. Finally wash with H₂O or buffer. Collect beads by filtration, dry and store in the refrigerator (4°C) under an inert gas.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Cleland’s REDUCTACRYL™ Reagent Certificates of Analysis

      TitleLot Number
      233157
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-April-2008 RFH
      SynonymsCleland’s Reagent, Immobilized
      DescriptionImmobilized form of DTT on polyacrylamide resin with applications similar to those of DTT itself (Cat. No. 233153 and 233155), but with the important advantage that no soluble components are added to the system. REDUCTACRYL™ Reagent can be used in a batch technique, in which the resin can be easily removed by filtration, or it can be packed into a column. Using a 10-fold excess of resin in the batch technique it has been shown that 1 mM oxidized glutathione can be reduced completely to glutathione in 15 min at room temperature, pH 7.5. Has a reduction capacity of >0.05 mEq/g resin. Can be used in either aqueous or organic solvent systems such as DMSO or ethanol.
      FormOff-white solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number3483-12-3
      RTECSEK1610000
      SolubilityAqueous buffers, DMSO, or Methanol
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Special InstructionsUnstable in solution; reconstitute just prior to use. To regenerate, expose to sodium borohydride (100-200 mg/g resin) in H₂O for 15 min. Follow by washing with H₂O containing 1 M acetic acid. Finally wash with H₂O or buffer. Collect beads by filtration, dry and store in the refrigerator (4°C) under an inert gas.
      Toxicity Harmful
      Merck USA index14, 3376