234153 Collagenase, Type I, Clostridium histolyticum

234153
  
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      Overview

      Replacement Information

      Key Spec Table

      Description
      Overview

      This product has been discontinued.



      Crude preparation that contains several collagenases, a sulfhydryl protease, clostripain, and trypsin-like proteolytic activities. Especially useful for tissue dissociation studies.

      Note: 1 ABC unit = 1.1 BTC unit.
      Catalogue Number234153
      Brand Family Calbiochem®
      References
      ReferencesHarris, E.D., Jr., and Krane, S.M. 1974. N. Engl. J. Med. 291, 557.
      Seifter, S., and Harper, E. 1970. Methods Enzymol. 19, 613.
      Product Information
      CAS number9001-12-1
      Activity≥125 ABC units/mg solid
      Unit of DefinitionOne ABC unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent per min from collagen at 37°C, pH 7.2. Note: 1 ABC unit = 1.1 BTC (bovine tendon collagen) unit.
      EC number3.4.24.3
      FormLyophilized
      Hygroscopic Hygroscopic
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      ContaminantsCaseinase activity: ≤100 FFC unit/500 ABC unit
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38-42/43

      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 22-24-37

      Do not breathe dust.
      Avoid contact with skin.
      Wear suitable gloves.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Collagenase, Type I, Clostridium histolyticum SDS

      Title

      Safety Data Sheet (SDS) 

      Collagenase, Type I, Clostridium histolyticum Certificates of Analysis

      TitleLot Number
      234153

      References

      Reference overview
      Harris, E.D., Jr., and Krane, S.M. 1974. N. Engl. J. Med. 291, 557.
      Seifter, S., and Harper, E. 1970. Methods Enzymol. 19, 613.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-February-2017 JSW
      DescriptionCrude enzyme preparation that contains several collagenases, a sulfhydryl protease, clostripain and trypsin-like proteolytic activities. Especially useful for tissue dissociation studies. Most active on fat cells, adrenal and liver tissue, with an optimal pH of 7.0-8.0.
      FormLyophilized
      CAS number9001-12-1
      EC number3.4.24.3
      ContaminantsCaseinase activity: ≤100 FFC unit/500 ABC unit
      Activity≥125 ABC units/mg solid
      Unit definitionOne ABC unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent per min from collagen at 37°C, pH 7.2. Note: 1 ABC unit = 1.1 BTC (bovine tendon collagen) unit.
      SolubilityAqueous buffers (1 mg/ml)
      Storage +2°C to +8°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
      Toxicity Harmful
      Merck USA index14, 2481
      ReferencesHarris, E.D., Jr., and Krane, S.M. 1974. N. Engl. J. Med. 291, 557.
      Seifter, S., and Harper, E. 1970. Methods Enzymol. 19, 613.