411493 Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6023) Biotin Conjugate

411493
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Catalogue Number411493
      Brand Family Calbiochem®
      References
      ReferencesHamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
      Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
      Jefferis, R., et al. 1986. Immunol. Lett. 10, 223.
      Product Information
      FormLiquid
      FormulationIn PBS, 1% BSA, pH 7.4.
      PreservativeNone
      Applications
      Application ReferencesSpecificity by ELISA, chimeric antibodies Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Specificity by ELISA, human myeloma proteins Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Jefferis, R., et al. 1986. Immunol. Lett. 10, 223. Enzyme Immunoassay Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
      Key Applications Enzyme Immunoassay
      Application NotesEnzyme Immunoassay (1:1000, see application references)
      Application CommentsAntibody should be titrated for optimal results in individual systems.

      Cross-reactivity by ELISA against human myeloma proteins:
      Human IgG1: < 0.5%
      Human IgG2: < 0.1%
      Human IgG3: < 0.5%
      Human IgG4: 100%
      Human IgM: < 0.1%
      Human IgA: < 0.1%
      Human IgE: < 0.1%

      Cross-reactivity by ELISA against chimeric antibodies:
      Human IgG1: < 0.01%
      Human IgG2: < 0.01%
      Human IgG3: < 0.01%
      Human IgG4: 100%
      Human IgM: < 0.01%
      Human IgA: < 0.01%
      Human IgE: < 0.01%
      Biological Information
      ImmunogenHuman
      CloneHP6023
      HostMouse
      IsotypeIgG₃
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Do not freeze/thaw more than 5 times.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6023) Biotin Conjugate Certificates of Analysis

      TitleLot Number
      411493

      References

      Reference overview
      Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
      Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
      Jefferis, R., et al. 1986. Immunol. Lett. 10, 223.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-August-2007 RFH
      ApplicationEnzyme Immunoassay (1:1000, see application references)
      DescriptionPurified mouse monoclonal antibody conjugated to biotin. Recognizes human IgG4 Fc fragment.
      BackgroundThe HP series of murine human Ig-specific monoclonal antibodies was produced from documented hybridoma cell lines that were developed at the Centers for Disease Control in Atlanta, Georgia. Laboratory analysis of each monoclonal antibody lot is performed in two stages during the production of ascites. First, the cell culture medium is analyzed for the presence and relative amount of human Ig-specific antibody by ELISA before injection of cells into mice. Second, collected ascites are analyzed for potency and antibody specificity using ELISA and isoelectric focusing (IEF), often in combination with an affinity immunoblot. For more information, the Calbiochem® technical monograph, The Human IgG Subclasses, is available free of charge.
      HostMouse
      Immunogen speciesHuman
      CloneHP6023
      IsotypeIgG₃
      FormLiquid
      FormulationIn PBS, 1% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.

      Cross-reactivity by ELISA against human myeloma proteins:
      Human IgG1: < 0.5%
      Human IgG2: < 0.1%
      Human IgG3: < 0.5%
      Human IgG4: 100%
      Human IgM: < 0.1%
      Human IgA: < 0.1%
      Human IgE: < 0.1%

      Cross-reactivity by ELISA against chimeric antibodies:
      Human IgG1: < 0.01%
      Human IgG2: < 0.01%
      Human IgG3: < 0.01%
      Human IgG4: 100%
      Human IgM: < 0.01%
      Human IgA: < 0.01%
      Human IgE: < 0.01%
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Do not freeze/thaw more than 5 times.
      Toxicity Standard Handling
      ReferencesHamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
      Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
      Jefferis, R., et al. 1986. Immunol. Lett. 10, 223.
      Application referencesSpecificity by ELISA, chimeric antibodies Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Specificity by ELISA, human myeloma proteins Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Jefferis, R., et al. 1986. Immunol. Lett. 10, 223. Enzyme Immunoassay Hamilton, R.G. 1987. Clin. Chem. 33, 1707.