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69090 pIE1-3 DNA

69090
  
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Overview

Replacement Information
Description
OverviewThe pIE1-3 vector is designed for constitutive expression of recombinant proteins from the baculovirus ie1 promoter in stably-transformed insect cells (1). The plasmid contains all promoter sequences known to be important for ie1-mediated gene expression in uninfected insect cells as well as the hr5 enhancer element. pIE1-3 lacks an ie1-driven translation initiation site; therefore, the ATG start codon must be supplied by the insert. To obtain stably-transformed cells, a pIE1-3 recombinant is cotransfected with pIE1-neo, which contains the Neo selectable marker under ie1 control.
Catalogue Number69090
Brand Family Novagen®
References
References1. Jarvis, D.L., Weinkauf, C. and Guarino, L.A. (1996) Prot. Exp. Pur. 8, 191–203.
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice or Dry Ice
Toxicity Standard Handling
Storage FREEZER (-20°C)
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69090 0

Documentation

pIE1-3 DNA Certificates of Analysis

TitleLot Number
69090

References

Reference overview
1. Jarvis, D.L., Weinkauf, C. and Guarino, L.A. (1996) Prot. Exp. Pur. 8, 191–203.

Citations

Title
  • Katrin Schnizler, et al. (2005) A novel chloride channel in Drosophila melanogaster is inhibited by protons. Journal of Biological Chemistry 28o, 16254-16262.