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S0902

SSC Buffer

Name: SSC Buffer
Brand: SIGMA

for Northern and Southern blotting, powder blend

동의어(들):

Saline-sodium citrate buffer

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크기 선택

보기 변경

제품정보 (DICE 배송 시 비용 별도)

NACRES:

NA.25

UNSPSC Code:

41105319

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도움 문의

속성

Quality Segment

200

form

powder blend

pH

6.8-7.2 (20-25 °C, 263 mg/L in water)

solubility

water: 263 mg/mL, clear, colorless

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

storage temp.

room temp

설명

General description

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Application

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.

Suitable for:

Preparing nucleic acids for hybridization
Hybridization buffer for Northern and Southern blots
Washing buffer for Northern and Southern blots

Features and Benefits

Multi-purpose use in hybridizations
Easily reconstituted and diluted to any concentration needed

Preparation Note

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

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저장 등급

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c