N2765

4-Nitrophenyl phosphate disodium salt hexahydrate

Name: 4-Nitrophenyl phosphate disodium salt hexahydrate
Brand: SIGMA

chromogenic, tablet

Synonym(s):

p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate

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All Photos(2)

About This Item

Linear Formula:

O₂NC₆H₄OP(O)(ONa)₂· 6H₂O

CAS Number:

333338-18-4

Molecular Weight:

371.14

NACRES:

NA.83

PubChem Substance ID:

24897592

UNSPSC Code:

12352204

EC Number:

224-246-5

MDL number:

MFCD00066288

Beilstein/REAXYS Number:

5324661

Product Name

4-Nitrophenyl phosphate disodium salt hexahydrate, tablet

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

form

tablet

solubility

water, high purity: 0.25 tablet/mL, clear, colorless

storage temp.

−20°C

Quality Level

300

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Related Categories

Chemistry & Biochemicals

Phosphates

Protein Biology

Proteins & Enzymes

Detection Substrates & Enzymes

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:

as a substrate for alkaline phosphatase coupled anti-rabbit antibody in enzyme-linked immunosorbent assay (ELISA)
as a substrate for the mouse secondary antibody linked to alkaline phosphatase in immunoenzymometric assay
in ELISA to analyze the presence of antibodies to Pseudomonas aeruginosa in animal serum sample

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Preparation Note

Contains 20 mg substrate per tablet

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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